Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Puerariae flos inhibits inflammatory responses in interferon-γ and lipopolysaccharide-stimulated mouse peritoneal macrophages

  • Hong, Seung-Heon (College of Pharmacy, Wonkwang University) ;
  • Kim, Hong-Joon (Department of Oriental Pharmacy, College of Pharmacy, Woosuk University) ;
  • Cha, Dong-Seok (Department of Oriental Pharmacy, College of Pharmacy, Woosuk University) ;
  • Lee, Ju-Young (Department of Herb Science Shinsung College) ;
  • Na, Ho-Jeong (Department of Oriental Pharmacy, College of Pharmacy, Woosuk University)
  • Published : 2007.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In macrophages, nitric oxide (NO) is released as an inflammatory mediator and has been proposed to be an important modulator of many pathophysiological conditions including inflammation. In this study, we have examined the inhibition effects of NO production by 85% methanol extract of the flower of Pueraria thunbergiana (PF) in mouse macrophages. Extract of PF (1, 10, 100 ${\mu}g/ml$) inhibited NO production, inducible NO synthase and cyclooxygenase-2 expression in interferon-g and lipopolysaccharide-stimulated mouse peritoneal macrophages and it had no cytotoxicity. These data suggest that 85% methanol extract of PF might be useful in controlling macrophages mediated inflammatory disease.

Keywords

References

  1. Adams DO, Hamilton TA. (1984) The cell biology of macrophage activation. Annu. Rev. Immunol. 2,283-318 https://doi.org/10.1146/annurev.iy.02.040184.001435
  2. Ambs S, Merriam WG, Bennett WP, Felley-Bosco E, Ogunfusika MO, Oser SM, Klein S, Shields PG, Billiar TR, Harris CC. (1998) Frequent nitric oxide synthase-2 expression in human colon adenomas: implication for tumor angiogenesis and colon cancer progression. Cancer Res. 58, 334-341
  3. Blackman DJ, Morris-Thurgood JA, Atherton JJ, Ellis G.R, Anderson RA, Cockcroft JR, Frenneaux MP. (2000) Endothelium-derived nitric oxide contributes to the regulation of venous tone in humans. Circulation 18,165-170
  4. Chung HS, Jeong HJ, Hong SH, Kim MS, Kim SJ, Song BK, Jeong IS, Lee EJ, Ann JW, Baek SH. Kim HM. (2002) Induction of nitric oxide synthase by Oldenlandia diffusa in mouse peritoneal macrophages. Biol. Pharm. Bull. 25,1142-1146 https://doi.org/10.1248/bpb.25.1142
  5. Goldstein SR, Yang GY, Chen X, Curtis SK, Yang CS. (1998) Studies of iron deposits, inducible nitric oxide synthase and nitrotyrosine in a rat model for esophageal adenocarcinoma. Carcinogenesis 19,1445-1449 https://doi.org/10.1093/carcin/19.8.1445
  6. Kanazawa K., Kawasaki H, Samejima K, Ashida H, Danno G. (1995) Specific desmutagen (antimutagen) in oregano against a dietary carcinogen, Trp-P-2, are galangin and quercetin. J. Agric. Food Chent. 43, 404-409 https://doi.org/10.1021/jf00050a028
  7. Koyanagi M, Egashira K, Kubo-Inoue M, Usui M, Kitamoto S, Tomita H, Shimokawa H, Takeshita A. (2000) Role of transforming growth factor-betal in cardiovascular inflammatory changes induced by chronic inhibition of nitric oxide synthesis. Hypertension 35, 86-90
  8. Keung WM, Vallee BL. (1998) Kudzu root: an ancient Chinese source of modern antidipsortropic agents. Phytochemistry 47, 499-506 https://doi.org/10.1016/S0031-9422(97)00723-1
  9. Minghetti L, Levi G. (1998) Microglia as effector cells in brain damage and repair: focus on prostanoids and nitric oxide. Prog. Neurobiol. 54, 99-125 https://doi.org/10.1016/S0301-0082(97)00052-X
  10. Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65,55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  11. Remick DG, Strieter R.M, Eskandari MK, Nguyen DT, Genord MA, Raiford CL, Kunkel SL. (1990) Role of tumor necrosis factor-alpha in lipopolysaccharide-induced pathologic alterations. Am. J. Pathol. 136, 49-60
  12. Sharma R, Coats AJ, Anker SD. (2000) The role of inflammatory mediators in chronic heart failure: cytokines, nitric oxide, and endothelin-1. Int. J. Cardiol. 15,175-186 https://doi.org/10.1016/S0167-5273(99)00186-2
  13. Szabo G. (2007) Moderate drinking, inflammation, and liver disease. Ann Epidemiol 17, S49-54 https://doi.org/10.1016/j.annepidem.2007.01.012
  14. Thiemermann C, Vane J. (1990) Inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharides in the rat in vivo. Eur. J. Pharmacol. 182, 591-595 https://doi.org/10.1016/0014-2999(90)90062-B
  15. Vilcek J, Lee TH. (1991) Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions. J. Biol. Chem. 266, 7313-7316
  16. Wallace JL. (1999) Distribution and expression of cyclooxygenase (COX) isoenzymes, their physiological roles, and the categorization of nonsteroidal antiinflammatory drugs (NSAIDs). Am. J. Med. 107, 11S-16S; discussion 16S-17S https://doi.org/10.1016/S0002-9343(99)00363-0
  17. Wilson KT, Fu S, Ramanujam KS, Meltzer SJ. (1998) Increased expression of inducible nitric oxide synthase and cyclooxygenase-2 in Barrett's esophagus and associated adenocarcinomas. Cancer Res. 58,2929-2934