Mass Propagation by In Vitro Culture of Bupleurum latissimum Nakai

섬시호(Bupleurum latissimum Nakai)의 조직배양을 통한 대량생산

This study was carried out to establish the micropropagation system of Bupleurum latissimum Nakai that is a Korean native endangered species. Callus were induced from the leaf, petiole and floral bud and the percentage of callus formation was highest in the floral bud on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D. Especially, callus induced from floral bud was formed 77.8% and the percentage of shoot formation was 42.6% on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ TDZ. For simultaneously callus formation and shoot regeneration, 1/2 MS medium was more effective than MS medium. The percentage callus formation, shoot regeneration and rooting were 46.3%, 13.0%, 13.0% in 1/2 MS medium, respectively. Soot regeneration from callus was good in 1/2 MS medium supplemented with 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ BA where percentage of shoot regeneration was 74.1 %, and the number of shoot per explant was 2.4. The percentage of rooting was lowest (57.8%) in control while it was highest (97.8%) in 1.5 mg ${\cdot}$ $L^{-1}$ NAA. In acclimatization of regenerated plantlets, the percentage of survived plantlets was highest (86.1%), and plant height, root length and fresh weight were good in the soil for horticulture.

엽, 엽병과 화뢰 등의 치상 절편체 부위 모두에서 캘러스가 형성되었으며 특히 화뢰에서 캘러스 형성률이 71.6%로 가장 높았다. 화뢰배양시 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ TDZ의 혼용처리구에서 캘러스 형성이 77.8%로 양호했으며 신초 재분화율 역시 42.6%를 보였다. 캘러스 형성과 신초 재분화를 동시에 시킬 때 1/2 MS배지가 효과적이었는데 1/2 MS배지에서 캘러스 형성률은 46.3%, 신초 재분화율은 13.0%,그리고 발근율은 13.0%를 보였다. 캘러스를 통한 신초의 재분화를 위해 1/2 MS배지에 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ BA를 첨가했을 때 신초 재분화율은 74.1%를 보였고 절편체당 신초수는 2.4개로 좋았다. 발근율은 대조구에서 57.8%로 낮았으나 1.5mg ${\cdot}$ $L^{-1}$ NAA 첨가된 배지에서 97.8%로 높았다. 재분화된 기내 식물체를 원예용 상토에 순화했을 때 생존률이 86.1%로 높았으며 초장, 근장, 생체중 역시 양호하였다.