Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-diabetic Effect of the Methanolic Extract of Ligularia stenocephala Leaves in the Streptozotocin-induced Rat

스트렙토조토신으로 유도된 흰쥐에서 곤달비 추출물의 항당뇨 효과

  • Published : 2007.08.30
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Abstract

The leaves of Ligularia stenocephala (Compositae) are used as a vegetable food to treat anxiety, weakness and menstrual disorder and are said to be effective against diabetic disease in Korea. This research was aimed to develop this vegetable food as a functional food to treat diabetic disease. Oral administration of MeOH extract exhibited significant hypoglycemic and hypolipidemic effects in streptozotocin (STZ)-induced diabetic rats at 100 and 200 mg/kg dose. This extract inhibited the increase of body weight, water consumption, food intake, blood glucose, lowered atherogenic index, and overcome glucose intolerance in the STZ-treated rat. These results suggest that the leaves of Ligularia stenocephala could be developed as a functional food with anti-diabetic effect.

곤달비 메탄올 추출물은 100mg/kg 및 200mg/kg의 경구투여로 STZ로 유도된 당뇨병의 제반 증상을 완화하였다. 즉, STZ의 투여는 흰쥐의 체중, 수분 섭취, 식이섭취를 증가시켰으며, 혈당량, 혈청 triglyceride 량, 동맥경화 위험지수를 증가시켰으나, 곤달비 추출물의 투여는 이러한 당뇨병에 관련된 증상을 개선하였다. 이와 더불어, 위의 당뇨병 모델 쥐에서 당내성 실험에서도 곤달비 추출물이 현저한 효과를 나타내었으므로 혈당의 세포내 이행을 촉진시키는 것으로 확인되었다. 이상과 같은 결과로부터 곤달비 추출물은 당뇨병 및 그에 따르는 고지혈증에 유효하였으므로 당뇨병 질환의 완화를 위하여 사용될 수 있는 산채 식품인 것으로 나타났다. 이러한 결과를 바탕으로 곤달비의 활성 물질의 입증과 그 이용에 관한 계속적인 연구가 필요한 것으로 생각된다.

Keywords

References

  1. Choi, E.M. 2007. Ligularia fischeri leaf extract prevents the oxidative streee in DBA/1J mice with type II collage-induced arthritis. Journal of Applied Toxicology 10: ahead of print
  2. Choi, J., J.K. Park, K.T. Lee, K.K. Park, W.B. Kim, J.H Lee, H.J. Jung and H.J. Park. 2004. Inhibitory Effect of Ligularia fischeri var. spiciformis and its Active Component, 3,4-Dicaffeoylquinic acid on the Hepatic Lipid Peroxidation in Acetaminophen-Treated Rat. Nat. Prod. Sci. 10: 182-189
  3. Choi, J., J.K. Park, K.T. Lee, K.K. Park, W.B. Kim, J.H. Lee, H.J Jung and H.J Park. 2005. In vivo antihepatotoxic effects of Ligularia fischeri var. spiciformis and the identification of the active component, 3,4-dicaffeoylquinic acid. J. Med. Food 8: 348-352 https://doi.org/10.1089/jmf.2005.8.348
  4. Friedewald, W.T., R.I. Levy and D.S. Fredrickson. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 24: 499- 502
  5. Hwang, B.Y., J.H. Lee, T.H. Koo, Y.S. Hog, J.S. Ro, K.S. Lee and J.J. Lee. 2002. Furanoligularenone, an eremophilane from Ligularia fischeri, inhibits the LPS-induced production of nitric oxide and prostaglandin E2 in macrophage RAW264.7 cells. Planta Med. 68: 101-105 https://doi.org/10.1055/s-2002-20250
  6. Kim, T.J. 1996. Korean Reources Plants, The Publishing Center of Seoul National University, Seoul pp. 242-243
  7. Ko, K.S. and E.S. Jeon. 2003. Ferns, Fern-Allies and Seed-bearing Plants of Korea, Iljin-Sa, Seoul pp. 680-681
  8. Jeong, S.H., S.J. Koo, J. Choi, H.J. Park, J. Ha and K.T. Lee. 2002. Intermedeol isolated from the leaves of Ligularia fischeri var. spiciformis induces the differentiation of human acute promyeolocytic leukemia HL-60 cells. Planta Med. 68: 881-885 https://doi.org/10.1055/s-2002-34932
  9. Lee, K.T., S.J. Koo, S.H. Jung, J. Choi, H.J. Jung and H.J. Park. 2002. Structure of three new terpenoids, spiciformisins a and b, and monocyclosqualene, isolated from the herbs of Ligularia fischeri var. spiciformis and cytotoxicity. Arch. Pharm. Res. 25: 820-823 https://doi.org/10.1007/BF02976998
  10. McGowan, M.W., J.D. Artiss, D.R. Strandbergh and B. Zak. 1983. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin. Chem. 29: 538-542
  11. Miller, N.E. 1978. The evidence for the antiatherogenicity of high density lipoprotein in man. Lipid. 13: 914-919 https://doi.org/10.1007/BF02533850
  12. Noma, A.,K. Nezu-Nakayama, M. Kita and H. Okabe. 1978. H Simultaneous determination of serum cholesterol in high- and lowdensity lipoproteins with use of heparin. $Ca^{2+}$, and an anion-exchange resin. Clin. Chem. 24: 1504-1508
  13. Park, H.J., S.H. Kwon, K.O. Yoo, I.C. Sohn, K.T. Lee and H.K. Lee. 2000. Sesquiterpenes from the leaves of Ligularia fischeri var. spiciformis. Planta Med. 66: 783-784 https://doi.org/10.1055/s-2000-9602
  14. Richmond, W. 1976. Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis. Clin. Chem. 22: 1579-1588
  15. Steinberg, D. 1997. Oxidative modification of LDL and atherogenesis. Circulation 95: 1062-1071 https://doi.org/10.1161/01.CIR.95.4.1062
  16. Toyoda, K., Y. Yaoita and M. Kikuchi. 2005. Three new dimeric benzofuran derivatives from the roots of Ligularia stenocephala Matsum. Et Koidz. Chem. Pharm. Bull. 53: 1555-1558 https://doi.org/10.1248/cpb.53.1555
  17. Wang, W.S., K. Gao and Z.J. Jia. 2000. Eremophilenolides from Ligularia fischeri. Planta Med. 66: 189-191 https://doi.org/10.1055/s-0029-1243131
  18. Yan, F.L., A.X. Wang and Z.J. Jia. 2005. Three new isopropenyl benzofurans from Ligularia stenocephala. Pharmazie 60: 155-159