The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of Kuwonsimsin-hwan on Immunosuppression Induced by Methotrexate in Rat

구원심신환(究原心腎丸)이 methotrexate로 유발된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

  • Choi, Soon-Ho (Dept. of Oriental Internal Medicine, Daegu Haany University, Korea Institute of Oriental Medicine) ;
  • Kang, Seok-Bong (Dept. of Oriental Internal Medicine, Daegu Haany University) ;
  • Choi, Sun-Mi (Korea Institute of Oriental Medicine) ;
  • Lee, Yeon-Kyeong (Dept. of Oriental Internal Medicine, Daegu Haany University)
  • 최순호 (대구한의대 한의과대학 신계내과교실, 한국한의학연구원) ;
  • 강석봉 (대구한의대 한의과대학 신계내과교실) ;
  • 최선미 (한국한의학연구원) ;
  • 이연경 (대구한의대 한의과대학 신계내과교실)
  • Published : 2007.06.30
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Abstract

Objectives : In the present study, the effect of Kuwonsimsin-hwan (KSS) was tested in methotrexate (MTX)-induced immunosuppressed SD rats. Methods : Methotrexate was fed to white rats once a day for 4 days. After the immune responses of the rats deteriorated, dried extracts of Kuwonsimsin-hwan mixed in water was fed to the rats once a day for 14 days. We then measured the number of lymphocytes in peripheral blood and the percentage of B-cells, T-cells, CD3+CD4+T-cells, CD3+CD8+ T-cells and IL-2 productivity sampled from spleen and peripheral region. Results : (1) The number of lymphocytes and the percentage of T-cells and CD3+CD4+ T-cellsin peripheral blood increased significantly in the KSS group as compared with the control group. (2) The percentage of B-cells, CD3+CD8+ T-cells, and CD4+/CD8+ T-cells in peripheral blood were not different statistically. (3) The percentage of T-cells in spleen and IL-2 productivity of spleen cells increased significantly in the KSS group as compared with the control group. (4) The percentage of CD3+CD4+ T-cells in spleen increased in KSS the group as compared with the control group but without statistical significance. (5) The percentage of B-cells, CD3+CD8+ T-cells, and CD4+/CD8+ T-cells in spleen were not different statistically. Conclusion : It is concluded that Kuwonsimsin-hwan has immunostimulating effect on MTX-induced immunosuppressed SD rats.

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