Restorative Dentistry and Endodontics

  • 제32권5호
  • /
  • Pages.403-410
  • /
  • 2007
  • /
  • 2234-7658(pISSN)
  • /
  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
권호 표지
DOI QR코드

DOI QR Code

백서에서 치수 절단술 시행 시 Pro-Root® MTA 단독 사용군과 rhBMP-2 혼용 사용군 간의 조직 반응 비교 연구

Tissue response of Pro-Root® MTA with rhBMP-2 in pulpotomized rat teeth

  • 박경태 (울산대학교 의과대학 서울 아산병원 보존과) ;
  • 양원경 (울산대학교 의과대학 서울 아산병원 보존과) ;
  • 고현정 (울산대학교 의과대학 서울 아산병원 보존과) ;
  • 김미리 (울산대학교 의과대학 서울 아산병원 보존과)
  • Park, Kyung-Tae (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University) ;
  • Yang, Won-Kyung (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University) ;
  • Ko, Hyun-Jung (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University) ;
  • Kim, Mi-Ri (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University)
  • 발행 : 2007.09.29
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

이 실험의 목적은 백서에서 치수절단술을 시행시 MTA 시멘트 단독 사용군과 rhBMP-2 혼용 사용군 간의 조직 반응을 비교하기 위함이다. 32마리의 건강한 백서의 상악 양쪽 제 1 대구치를 사용하였다. 치수강을 개방한 후에 지혈을 시행하고 우측 제1 대구치에는 대조군으로 MTA 시멘트 1.2 g을 사용하여 치수절단면을 수복하였고, 좌측 제 1대구치에는 MTA 시멘트 1.2 g에 rhBMP-2 $1\;{\mu}g$을 첨가하여 치수절단면을 수복하였다. 그 후 광중합 글래스 아이오노머 시멘트로 충전하였다. 백서는 각 각 16마리씩 2주, 7주 뒤에 희생시키고, 병리조직표본을 제작하였다. 통계 처리는 SPSS-WIN 12.0 프로그램을 이용하여 student t-test를 시행하였다 (p < 0.05). 염증소견은 7주군보다 2주군에서 더 심하였으나, 통계적으로 유의하지는 않았다 또한 rhBMP-2를 첨가한 군이 MTA 단독 사용군보다 염증 소견이 적어 보였으나, 역시 통계적으로 유의하지는 않았다. 결론적으로 MTA 시멘트 치수 절단술 시행시 MTA 단독 사용군과 비교시 MTA 시멘트에 rhBMP-2의 첨가하는 것은 별다른 차이가 없었다.

The purpose of this study was to investigate whether rhBMP-2 (BMP2) could induce synergistic effect with $Pro-Root^{(R)}$ MTA (MTA) in pulpotomized teeth in the rats. Healthy upper first molars from thirty-two, 10 weeks old, Sprague-Dawley rats were used for this investigation. The molars were exposed with round bur, and light pressure was applied with sterilized cotton to control hemorrhage. 1.2 grams of MTA cement was placed in right first molars as a control group. In left first molars, $1\;{\mu}g$ of BMP2 was additionally placed on exposed pulps with MTA. All cavities were back-filled with light-cured glass-ionomer cements. The rats were sacrificed after 2 weeks and 7 weeks, respectively. Then histologic sections were made and assessed by light microscopy. Data were statistically analyzed via student t-test with SPSSWIN 12.0 program (p < 0.05). Inflammation observed in 2 weeks groups were severe compared to the 7 weeks groups. But the differences were not statistically significant. BMP2-addition groups had less inflammation than MTA groups in both periods, though these differences were also not statistically significant. In conclusion, the combination of BMP2 and MTA showed no differences with MTA only for pulpotomy of rat teeth.

키워드

참고문헌

  1. Schroder U. Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration. proliferation, and differentiation. J Dent Res 64:541-548, 1985 https://doi.org/10.1177/002203458506400407
  2. Tziafas D. The future role of a molecular approach to pulp-dentinal regeneration. Caries Res 38: 314-320, 2004 https://doi.org/10.1159/000077771
  3. Ranly DM. Garcia-Godoy FJ. Current and potential pulp therapies for primary and young permanent teeth. J Dent 28:153-161, 2000 https://doi.org/10.1016/S0300-5712(99)00065-2
  4. Torabinejad M. Chivian N. Clinical applications of mineral trioxide aggregate. J Endod 25:197-205. 1999 https://doi.org/10.1016/S0099-2399(99)80142-3
  5. Peng L, Ye L, Tan H, Zhou X. Evaluation of the formocresol versus mineral trioxide aggregate primary molar pulpotomy: a meta-analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102:40-4, 2006 https://doi.org/10.1016/j.tripleo.2005.02.067
  6. Abedi HR, Torabinejad M, Pitt Ford TR. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 127:1491-1494. 1996 https://doi.org/10.14219/jada.archive.1996.0058
  7. Pitt Ford TR, Torabinejad M, Abedi HR, Bakland LK, Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assac 127:1491-1494, 1996 https://doi.org/10.14219/jada.archive.1996.0058
  8. Faraco IM, Holland R. Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement. Dent Traumatol 17: 163-166, 2001 https://doi.org/10.1034/j.1600-9657.2001.170405.x
  9. Mitchell PJ, Pitt Ford TR. Torabinejad M, McDonald F. Osteoblast biocompatibility of mineral trioxide aggregate. Biomaterials 20:167-173, 1999 https://doi.org/10.1016/S0142-9612(98)00157-4
  10. Koh ET, McDonald F, Pitt Ford TR, Torabinejad M. Cellular response to Mineral Trioxide Aggregate. J Endod 24:543-547, 1998 https://doi.org/10.1016/S0099-2399(98)80074-5
  11. Koh ET, Torabinejad M, Pitt Ford TR, Brady K, McDonald F. Mineral trioxide aggregate stimulates a biological response in human osteoblasts. J Biomed Mater Res 37:432-439, 1997 https://doi.org/10.1002/(SICI)1097-4636(19971205)37:3<432::AID-JBM14>3.0.CO;2-D
  12. Torabinejad M, Rastegar A, Kattering J, Pitt Ford TR. Bacterial microleakage of mineral trioxide aggregate as a root-end filling material. J Endod 21: 109-112, 1995 https://doi.org/10.1016/S0099-2399(06)80433-4
  13. AI-Nazhan S, Al-Judai A. Evaluation of antifungal activity of mineral trioxide aggregate. J Endod 29:826-827, 2003 https://doi.org/10.1097/00004770-200312000-00010
  14. Al-Hezaimi K, AI-Shalan TA, Naghshbandi J, Oglesby S, Simon JH, Rotstein I. Antibacterial effect of two mineral trioxide aggregate(MTA) preparations against Enterococcus Faecalis and Streptococcus sanguis in vitro. J Endod 32:1053-1056, 2006 https://doi.org/10.1016/j.joen.2006.06.004
  15. Aeinehchi M, Eslami B,Ghanbariha M & Saffar AS. Mineral trioxide aggregate(MTA) and calcium hydroxide as pulp capping agents in human teeth: a preliminary report. Int Endod J 36:225-231, 2002 https://doi.org/10.1046/j.1365-2591.2003.00652.x
  16. Nakashima M, Akamine A. The application of tissue engineering to regeneration of pulp and dentin in endodontics. J Endod 31:711-718, 2005 https://doi.org/10.1097/01.don.0000164138.49923.e5
  17. Sloan AJ, Smith AJ. Stem cells and the dental pulp:potential roles in dentine regeneration and repair. Oral Dis 13: 151-157. 2007 https://doi.org/10.1111/j.1601-0825.2006.01346.x
  18. Wozney JM. The bone morphogenetic protein family: multifunctional cellular regulators in the embryo and adult. Eur J Oral Sci 106: 160-166. 1998
  19. Lianjia Y. Immunohistochemical localization of bone morphogenetic protein (EMP) in calcifying fibrous epulis. J Oral Pathol Med 22:406-410, 1993 https://doi.org/10.1111/j.1600-0714.1993.tb00131.x
  20. Tziafas D, Kolokuris I, Inductive influences of demineralized dentin and bone matrix on pulp cells: an approach of secondary dentinogenesis. J Dent Res 69:75-81. 1990 https://doi.org/10.1177/00220345900690011301
  21. Smith AJ, Tobias RS, Cassidy N, Begue-Kim C, Ruch JV, Lesot H. Influence of substrate nature and immobilization of implanted dentin matrix components during induction of reparative dentinogenesis. Connect Tissue Res 32:291-296, 1995 https://doi.org/10.3109/03008209509013736
  22. Nakashima M, Induction of dentin formation on canine amputated pulp by recombinant human bone morphogenetic proteins (BMP)-2 and -4, J Dent Res 73:1515-1522, 1994 https://doi.org/10.1177/00220345940730090601
  23. Canalis E, McCarthy T, Centrella M. Growth factors and the regulation of bone remodelling. J Clin Invest 81:277-281, 1988 https://doi.org/10.1172/JCI113318
  24. Hu CC, Zhang C, Quan Q, Tatum NB. Reparative dentin formation in rat molars after direct pulp capping with growth factors. J Endod 24:744-751. 1998 https://doi.org/10.1016/S0099-2399(98)80166-0
  25. Kim MR, Kim BH, Yoon SH. Effect on the healing of periapical perforations in dogs of the addition of growth factors to calcium hydroxide. J Endod 27: 734-737, 2001 https://doi.org/10.1097/00004770-200112000-00005
  26. Giannobile WV. Periodontal tissue engineering by growth factors. Bone 23S-37S, 1996 https://doi.org/10.1016/8756-3282(96)00107-X
  27. Nakashima M., Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix. Arch Oral Biol 39:1085-1089, 1994 https://doi.org/10.1016/0003-9969(94)90062-0
  28. Nakashima M, Reddi AH. The application of bone morphogenetic proteins to dental tissue engineering. Nat Biotechnol 21:1025-32, 2003 https://doi.org/10.1038/nbt864
  29. Begue-Kirm C, Smith AJ, Loriot M, Kupferle C, Ruch JV, Lesot H., Comparative analysis of TGF-${\beta}s$, BMPs, lGF, msxs, fibronectin, osteonectin and bone sialoprotein gene gene expression during normal and in vitro induced odontoblast differentiation. Int J Dev Biol 38 :405-20, 1994
  30. Torabinejad M, Kutsenko D, Machnick T, Ismail A, Newton CWo Levels of evidence for the outcome of nonsurgical endodontic treatment. J Endod 31 :637-646, 2005 https://doi.org/10.1097/01.don.0000153593.64951.14
  31. Iohara K, Nakashima M, Ito M, Ishikawa M, Nakashima A, Akamine A, Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2. J Dent Res 83: 590-595, 2004 https://doi.org/10.1177/154405910408300802
  32. Kim MR, Ko HJ, Yang WK, Kim WK, Lee YK. Cytotoxicity of ProRoot${\circleR}$ MTA cement with rhBMP-2. Journal of Korean Academics of Endodontics 7:39-49, 2006
  33. Six N, Lasfargues JJ, Goldberg M. Differential repair responses in the coronal and radicular areas of the exposed rat molar pulp induced by recombinant human bone morphogenetic protein 7 (osteogenic protein 1). Arch Oral Biol 47:177-187, 2002 https://doi.org/10.1016/S0003-9969(01)00100-5
  34. Goldberg M, Six N, Decup F, Lasfargues JJ, Salih E, Tompkins K, Veis A. Bioactive molecules and the future of pulp therapy. Am J Dent 16:66-76, 2003

피인용 문헌

  1. A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell vol.34, pp.5, 2009, https://doi.org/10.5395/JKACD.2009.34.5.415