Tissue response of Pro-Root® MTA with rhBMP-2 in pulpotomized rat teeth

백서에서 치수 절단술 시행 시 Pro-Root® MTA 단독 사용군과 rhBMP-2 혼용 사용군 간의 조직 반응 비교 연구

  • Park, Kyung-Tae (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University) ;
  • Yang, Won-Kyung (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University) ;
  • Ko, Hyun-Jung (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University) ;
  • Kim, Mi-Ri (Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University)
  • 박경태 (울산대학교 의과대학 서울 아산병원 보존과) ;
  • 양원경 (울산대학교 의과대학 서울 아산병원 보존과) ;
  • 고현정 (울산대학교 의과대학 서울 아산병원 보존과) ;
  • 김미리 (울산대학교 의과대학 서울 아산병원 보존과)
  • Published : 2007.09.29


The purpose of this study was to investigate whether rhBMP-2 (BMP2) could induce synergistic effect with $Pro-Root^{(R)}$ MTA (MTA) in pulpotomized teeth in the rats. Healthy upper first molars from thirty-two, 10 weeks old, Sprague-Dawley rats were used for this investigation. The molars were exposed with round bur, and light pressure was applied with sterilized cotton to control hemorrhage. 1.2 grams of MTA cement was placed in right first molars as a control group. In left first molars, $1\;{\mu}g$ of BMP2 was additionally placed on exposed pulps with MTA. All cavities were back-filled with light-cured glass-ionomer cements. The rats were sacrificed after 2 weeks and 7 weeks, respectively. Then histologic sections were made and assessed by light microscopy. Data were statistically analyzed via student t-test with SPSSWIN 12.0 program (p < 0.05). Inflammation observed in 2 weeks groups were severe compared to the 7 weeks groups. But the differences were not statistically significant. BMP2-addition groups had less inflammation than MTA groups in both periods, though these differences were also not statistically significant. In conclusion, the combination of BMP2 and MTA showed no differences with MTA only for pulpotomy of rat teeth.

이 실험의 목적은 백서에서 치수절단술을 시행시 MTA 시멘트 단독 사용군과 rhBMP-2 혼용 사용군 간의 조직 반응을 비교하기 위함이다. 32마리의 건강한 백서의 상악 양쪽 제 1 대구치를 사용하였다. 치수강을 개방한 후에 지혈을 시행하고 우측 제1 대구치에는 대조군으로 MTA 시멘트 1.2 g을 사용하여 치수절단면을 수복하였고, 좌측 제 1대구치에는 MTA 시멘트 1.2 g에 rhBMP-2 $1\;{\mu}g$을 첨가하여 치수절단면을 수복하였다. 그 후 광중합 글래스 아이오노머 시멘트로 충전하였다. 백서는 각 각 16마리씩 2주, 7주 뒤에 희생시키고, 병리조직표본을 제작하였다. 통계 처리는 SPSS-WIN 12.0 프로그램을 이용하여 student t-test를 시행하였다 (p < 0.05). 염증소견은 7주군보다 2주군에서 더 심하였으나, 통계적으로 유의하지는 않았다 또한 rhBMP-2를 첨가한 군이 MTA 단독 사용군보다 염증 소견이 적어 보였으나, 역시 통계적으로 유의하지는 않았다. 결론적으로 MTA 시멘트 치수 절단술 시행시 MTA 단독 사용군과 비교시 MTA 시멘트에 rhBMP-2의 첨가하는 것은 별다른 차이가 없었다.


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