Journal of Radiation Protection and Research

The Korean Association for Radiation Protection (대한방사선방어학회)
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The Effect of Bamboo (Phyllostachys nigra var. henenis Strapf) Leaf Extract on Epidermal Melanocytes in Ultraviolet B-irradiated Mice

자외선 B를 조사한 마우스 표피멜라닌세포 변화에 대한 분죽(Phyllosrachys nigra var. henenis Strapf)잎 추출물의 효과

  • Lee, Hae-June (College of Veterinary Medicine, Chonnam National University) ;
  • Chae, Se-Lim (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Published : 2007.06.30
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Abstract

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of bamboo (Phyllostachys nigra var. henenis Strapf) leaf extract (BLE) on the formation, and decrease of UVB-induced epidermal melanocytes. C57BL/6 mice were irradiated by $UVB\;80mJ/cm^2(0.5mW/sec)$ daily for 7 days, and BLE was intraperitoneally or topically applied pre-or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 $melanocytes/mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with BLE before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with BLE at 3rd and 6th weeks after irradiation. The results of present study indicate that BLE is likely to be useful as inhibitor of UVB-induced pigmentation and depigmenting agent.

C57BL/6 마우스에서 자외선 B(UVB)조사에 의한 표피 멜라닌세포의 변화에 대한 대나무(분죽, Phyllostachys nigra var. henenis Suapf ) 잎 추출물 (BLE)의 효과를 관찰하였다. 마우스에 UVB를 매일 $80mJ/cm^2(0.5mW/sec)$씩 7일간 조사하고 BLE를 UV조사 전 또는 조사 후에 복강내주사 또는 피부에 도포하여 멜라닌세포 형성 억제효과 및 형성된 멜라닌세포에 대한 미백효과를 dihydroxyphenylalanine (DOPA) 염색으로 관찰하였다. 마우스의 귀등쪽 표피를 분리하여 관찰한바, 정상대조군에서는 $mm^2$당 11-16개의 멜라닌세포가 관찰되었으며, UV조사 일주일 후 발달된 가지를 가진 DOPA 양성 멜라닌세포는 급격히 증가하였다. 멜라닌세포 형성 억제 실험에서는 평균치를 기준으로 복강내 주사군에서 29.9%, 피부도포군에서 33.3%의 유의성 있는 억제 효과가 관찰되었으며, 형성된 멜라닌세포의 감소 효과 실험에서는 평균치를 기준으로 복강내 주사군의 경우 6주에 24.4%의 감소 효과가 관찰되었고, 피부도포군의 경우 3주에 21.0%, 6주에 10.4%의 유의성 있는 감소 효과를 보였다. 이상의 결과는 BLE가 UV에 의한 멜라닌세포 형성 억제제 및 미백제로서의 적용 가능성을 제시하였다.

Keywords

References

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