Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Effect of Progesterone on Cultured Human Dermal Fibroblast

배양된 인체진피섬유모세포의 증식에 대한 황체호르몬의 영향

  • Kwon, Soon Sung (Research Institute of Plastic and Reconstructive Surgery) ;
  • Oh, Myung June (Research Institute of Plastic and Reconstructive Surgery) ;
  • Lee, Jin Hee (Research Institute of Plastic and Reconstructive Surgery) ;
  • Park, Jong Lim (Research Institute of Plastic and Reconstructive Surgery) ;
  • Chang, Hak (Research Institute of Plastic and Reconstructive Surgery) ;
  • Minn, Kyung Won (Research Institute of Plastic and Reconstructive Surgery)
  • 권순성 (서울대학교병원 성형재건연구소) ;
  • 오명준 (서울대학교병원 성형재건연구소) ;
  • 이진희 (서울대학교병원 성형재건연구소) ;
  • 박종림 (서울대학교병원 성형재건연구소) ;
  • 장학 (서울대학교병원 성형재건연구소) ;
  • 민경원 (서울대학교병원 성형재건연구소)
  • Received : 2006.11.29
  • Published : 2007.07.10
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Abstract

Purpose:The mechanism of scar formation is not fully understood. Fibroblast is an important cell in wound healing process. We experienced a patient who was taking progesterone orally. Upper blepharoplasty was performed on her but, wound healing was delayed. We hypothesized that progesterone was the cause of delayed wound healing and fibroblast proliferation inhibition. We investigated the effect of progesterone in vitro on human dermal fibroblasts to study the effects on fibroblast proliferation. Methods: Human dermal fibroblasts from four persons were cultured initially. Progesterone is mixed to them at various concentrations, and fibroblast cell count was measured by MTT assay method at 570 nm. We confirmed that progesterone has some inhibitory effect on fibroblast proliferation and maximal inhibitory concentration of progesterone was determined. Then fibroblasts from a total of nineteen persons were cultured and the effects of progesterone were studied. Results: The initial study showed the maximal inhibitory concentration of progesterone to be $50{\mu}g/ml$. The main study showed that progesterone had 70.9% inhibitory effect on human dermal fibroblast in vitro. Conclusion: Progesterone has inhibitory effect on cultured human dermal fibroblast proliferation in vitro.

Keywords

Acknowledgement

Supported by : 한국과학재단

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