Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
권호 표지

Effect of Transplantation of Human Bone Marrow Stromal Cells or Dermal Fibroblasts on Wound Healing

인간 골수기질세포 또는 진피섬유모세포의 이종이식이 흰쥐의 창상치유속도에 미치는 영향

  • Han, Seung-Kyu (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Choi, Won Il (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Lee, Byung Il (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Kim, Woo Kyung (Department of Plastic Surgery, Korea University College of Medicine)
  • 한승규 (고려대학교 의과대학 성형외과학교실) ;
  • 최원일 (고려대학교 의과대학 성형외과학교실) ;
  • 이병일 (고려대학교 의과대학 성형외과학교실) ;
  • 김우경 (고려대학교 의과대학 성형외과학교실)
  • Received : 2007.01.22
  • Published : 2007.07.10
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The aim of this study is to compare the effects of bone marrow stromal cells(BSCs) and fibroblasts on wound healing activity in vivo, especially on epithelization. Methods: The fibroblasts and BSCs were harvested from patients and cultured. Ten Spague-Dawley white rats were used. A 5 mm punches were made to excise skin and subcutaneous tissue in a round fashion at six sites on the back area of each rat. Four hundred thousand cells suspended in 0.05 ml fibrinogen were applied to the created wounds. The cells in group I, II, and III were no cells, fibroblasts and BSCs. The lengths of epithelial gap at the widest wound site were compared with autopsy specimens obtained on the 6th day after cell therapy under light microscope. Statistical comparisons were performed using the Mann-Whitney U-test, and the p value < 0.05 was considered statistically significant. Results: The best epithelization was also seen in the BSC group, followed by fibroblast and no cell groups.Conclusion: These results demonstrate that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

Keywords

Acknowledgement

Supported by : 보건복지부

References

  1. Kuroyanagi Y, Yamada N, Yamashita R, Uchinuma E: Tissue-engineered product: Allogenic cultured dermal substitute composed of spongy collagen with fibroblasts. Artif Organs 25: 180, 2001 https://doi.org/10.1046/j.1525-1594.2001.025003180.x
  2. Gohari S, Gambla C, Healey M, Spaulding G, Gordon KB, Swan J, Cook B, West DP, Lapiere JC: Evaluation of tissue-engineered skin (human skin substitute) and secondary intention healing in the treatment of full thickness wounds after Mohs micrographic or excisional surgery. Dermatol Surg 28: 1107, 2002 https://doi.org/10.1046/j.1524-4725.2002.02130.x
  3. Conget PA, Minguell JJ: Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 181: 67, 1999 https://doi.org/10.1002/(SICI)1097-4652(199910)181:1<67::AID-JCP7>3.0.CO;2-C
  4. Quirici N, Soligo D, Bossolasco P, Servida F, Lumini C, Deliliers GL: Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp Hematol 30: 783, 2002 https://doi.org/10.1016/S0301-472X(02)00812-3
  5. Han SK, Yoon TH, Lee DG, Lee MA, Kim WK: Potential of human bone marrow stromal cells to accelerate wound healing in vitro. Ann Plast Surg 55: 414, 2005 https://doi.org/10.1097/01.sap.0000178809.01289.10
  6. Pierce GF: Inflammation in nonhealing diabetic wounds: the space-time continuum does matter. Am J Pathol 159: 399, 2001 https://doi.org/10.1016/S0002-9440(10)61709-9
  7. Schultz GS, Sibbald RG, Falanga V, Ayello EA, Dowsett C, Harding K, Romanelli M, Stacey MC Teot L, Vanscheidt W: Wound bed preparation: a systematic approach to wound management. Wound Repair Regen 11: 1, 2003 https://doi.org/10.1046/j.1524-475X.2003.11101.x
  8. Han SK, Choi KJ, Kim WK: Clinical application of fresh fibroblast allografts for the treatment of diabetic foot ulcers: a pilot study. Plast Reconstr Surg 114: 1783, 2004 https://doi.org/10.1097/01.PRS.0000142415.57470.DF
  9. Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276: 71, 1997 https://doi.org/10.1126/science.276.5309.71
  10. Han SK, Chun KW, Kye MS, Kim WK: The effect of transplantation of human mesenchymal stem cells and dermal fibroblasts on the angiogenesis in rats. J Korean Soc Plast Reconstr Surg 31: 539, 2004
  11. Fathke C, Wilson L, Hutter J, Kapoor V, Smith A, Hocking A, Isik F: Contribution of bone marrow-derived cells to skin: collagen deposition and wound repair. Stem Cells 22: 812, 2004 https://doi.org/10.1634/stemcells.22-5-812
  12. Bi Y, Stuelten CH, Kilts T, Wadhwa S, Iozzo RV, Robey PG, Chen XD, Young MF: Extracellular matrix proteoglycans control the fate of bone marrow stromal cells. J Biol Chem 26: 30481, 2005
  13. Abe R, Donnelly SC, Peng T, Bucala R, Metz CN: Peripheral blood fibrocytes: differentiation pathway and migration to wound sites. J Immunology 166: 7556, 2001 https://doi.org/10.4049/jimmunol.166.12.7556
  14. Sen CK, Khanna S, Babior BM, Hunt TK, Ellison EC, Roy S: Oxidant-induced vascular endothelial factor expression in human keratinocytes and cutaneous wound healing. J Biol Chem 277: 33284, 2002 https://doi.org/10.1074/jbc.M203391200
  15. Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E: Defining the epithelial stem cell niche in skin. Science 303: 359, 2004 https://doi.org/10.1126/science.1092436