• Title/Summary/Keyword: Progesterone

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Immunohistochemical application of anti-proliferating cell nuclear antigen antibody on the development of uterus following sex hormone administration in rats (성 hormone이 rat 자궁 발달에 미치는 영향에 대한 proliferating cell nuclear antigen 항체의 면역조직학적 응용)

  • Koh, Phil-ok;Kwak, Soo-dong
    • Korean Journal of Veterinary Research
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    • v.37 no.2
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    • pp.281-289
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    • 1997
  • The study was designed to investigate the effects of progesterone and estrogen on the uterus of rats by immunohistochemical methods using Proliferating Cell Nuclear Antigen (PCNA) antibody. Eighteen female rats(Wistar), weighing initially about 300g, were ovariectomized. These rats were divided into four groups, progesterone-treated group, estrogen-treated group, estrogen+progesterone-treated group, and control group, progesterone-treated group was injected with 1mg of progesterone per rat per day for 2 days and estrogen-treated group with $20{\mu}g$ of $17{\beta}-estradiol$ for 3 days and estrogen+progesterone-treated group with $17{\beta}-estrdiol$ for 3 days and then with progesterone for 2 days as above. In gross findings, the uteri were markedly hypertrophied by estrogen treatment but were not affect in size by progesterone treatment. Immunohistochemical investigation was performed on the cell types with higher appearance of PCNA positive reaction cells in four groups. The groups with higher appearance of the stromal cells were ordered as estrogen-treated group, progesterone-treated group, estrogen+progesterone-treated group, and control group. The muscle cells were ordered as progesterone-treated group, estrogen-treated group, estrogen+progesterone-treated group, and control group. Positive reaction cells of the stromal cells were total 4.6 times higher than those of muscle cells. Therefore, the affect of the hypertrophy on the uterus by estrogen was larger than those of progesterone and affect on the uterus by stromal cells were larger than those of muscle cells. The group with more PCNA positive reaction cells of luminal epithelial cells were ordered as control group, progesterone-treated group, estrogen+progesterone-treated group, and estrogen-treated group, and glandular epithelial cells were ordered as estrogen+progesterone-treated group, progesterone-treated group, control group, and estrogen-treated group. It was suggested that estrogen and progesterone did not affect on the proliferating cells of luminal epithelial cells and affection of progesterone on the development of glandular epithelial cell was larger than that of estrogen.

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Effect of Progesterone on the Metabolism of Washed Goat Spermatozoa (산양정자의 대사에 미치는 Progesterone의 영향)

  • ;J. Macebi
    • Korean Journal of Animal Reproduction
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    • v.9 no.1
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    • pp.62-65
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    • 1985
  • This study was conducted to investigate the effect of progesterone on the oxygen consumption of washed goat spermatozoa. The results obtained were summarized as follows: 1. Progesterone significantly depressed the respilation of the spermatozoa. 2. Caffeine and Di-cAMP greatly increased the oxygen consumption of the spermatozoa. 3. Caffeine plus progesterone and Di-cAMP plus progesterone significantly depressed the oxygen uptake of the cells. 4. There is some indication of a relationship between progesterone and its interference with metabolic behavior of sperm.

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Controlled Release of Progesterone from Polyethylene Oxide-Silicone Rubber Matrix

  • Kim, Sung-Ho;O, Sung-l
    • Archives of Pharmacal Research
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    • v.12 no.3
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    • pp.191-195
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    • 1989
  • The release of progesterone from monolithic devices composed of different ratios of polyethylene oxide (PEO; mw 20, 000) and hydrophobic polydimethylsiloxane was investigated. Water soluble PEO soaked into the polymer provided controlled release of progesterone. The release rate of progesterone could be controlled by varying the contents of PEO and progesterone in soaking solution. The progesterone release rate from silicone devices increased as the content of PEO in devices increased, while it decreased as the content of PEO in soaking solution increased. The release rate may be made by simple alterations of geometry of devices controlled swelling and the change in the physical structure of polymer network. Hydrophobic polydimethylsiloxane containing PEO and progesterone can provide a contraceptive material for prolonged release of progesterone.

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Enhancement of Biotransformation Yield in 11$\alpha$-Hydroxylation of Progesterone by Continuous Addition of the Substrate (Progesterone의 연속첨가에 의한 11$\alpha$-hydroxyprogesterone으로의 생물전환수율의 증대)

  • 최용복;김학성;박영훈
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.280-285
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    • 1990
  • Biotransformation of progesterone to 11 $\alpha$ -hydroxyprogesterone by growing cells of Rhizopus nigricans was investigated. As the concentration of progesterone increased, the specific growth rate of R. nigricans decreased linearly, and consequently the conversion yield lowered. The hyphae of the microorganism were observed to become thicker, shorter, and more densely branched at high concentrations of progesterone. In order to improve the process productivity, biotransformation was conducted with continuous addition of progesterone. When the substrate was added continuously at a rate of 0.86 g/hr for 30 hrs, overall conversion yield reached upto 56% while a single addition of the same amount of progesterone yielded about 40% eonversion. When additional feeding of glucose was carried out upon its depletion, an improved br'oconversion yield upto 68% was obtained.

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Progesterone assays as an aid for improving reproductive efficiency in dairy cattle III. Milk progesterone profiles in repeat-breeder dairy cows (Progesterone 농도측정(濃度測定)에 의한 유우(乳牛)의 번식효율증진(繁殖效率增進)에 관한 연구(硏究) III. 저수태우(低受胎牛)에서 유즙(乳汁)중 progesterone 농도변화(濃度變化))

  • Kang, Byong-kyu;Choi, Han-sun;Choi, Sang-gong;Son, Chang-ho;Chon, Hong-suk
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.189-193
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    • 1994
  • This study was undertaken to clarify the relationship between the cause of repeat-breeder and the luteal dysfunction in repeat-breeder dairy cows that failed to conceive to three or more artificial insemination(AI) at a regular interval. Progesterone concentrations were measured in milk fat for 20 to 22 days after AI. From the 15 repeat-breeder dairy cows, six cows had a normal progesterone profiles. Five cows showed a delayed rise of the progesterone concentrations until 7 to 10 days after AI, two cows had a comparatively low concentration of milk progesterone below 150 ng/ml through most of the luteal phase, and two cows had a combined pattern of a delayed rise and a low concentration of milk progesterone during luteal phase. It is suggest that luteal dysfunction as indicated by progesterone profiles is one of the causes of repeat-breeder in dairy cows.

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Effects of Progesterone on the Macromolecular Syntheses in Mouse Preimplantation Embryos in Vitro (프로제스트론이 培養中인 생쥐 初期胚兒의 高分子化合物合成에 미치는 影響에 관하여)

  • Cho, Wan-Kyoo;Kwon, Hyuk-Bang
    • The Korean Journal of Zoology
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    • v.22 no.2
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    • pp.81-93
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    • 1979
  • Metabolic changes of early mouse embryos treated with progesterone were investigated in order to elucidate the mode of action of progesterone on embryogenesis in vitro. The embryos were cultured, and labelled with radioactive precursors of macromolecules for certain periods in the absence or presence of various concentrations of progesterone by employing the microtube culture technique. The changes of transport and macromolecular synthesis systems of the embryos were examined by measuring the amounts of uptake and incorporation of the precursors. The results obtained were as follows: 1. Progesterone stimulated markedly the uptake of amino acids, but rather suppressed their incorporation by embryonic cells. 2. Progesterone suppressed both the uptake and incorporation of nucleotide precursors (uridine and thymidine) by embryonic cells. 3. Progesterone penetrated into the embryonic cell membranes and was taken up by them. The present results seem to indicate that the inhibition of the progesterone on the mammalian embryogenesis in vitro may not be directly related to the membrane transport system. They seem to imply that progesterone would penetrate into the embryonic cells and may directly block the biosynethetic pathways of macromolecules, and so lead to the inhibition of the embryogenesis in vitro.

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Studies on Changes of Progesterone and $20\alpha$-Dihydroprogesterone Levels in Serum by Progesterone-tube Implantation during Pregnancy in Rats (흰쥐의 번식과정에 있어서 Progesterone-tube 이식이 혈청내 Progesterone과 $20\alpha$-Dihydroprogesterone 수준변화에 미치는 영향)

  • 민관식;오석두;윤창현
    • Korean Journal of Animal Reproduction
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    • v.14 no.4
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    • pp.273-280
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    • 1990
  • This study was conducted to find out the changes of progesterone and 20$\alpha$-dihydroprogesterone(OHP) levels in the serum of female rats by progesterone-tube implantation during late pregnancy. One hundred and twenty rats, 10-13 weeks old, were offered for this experiment. Blood samples were taken from the rats on 18, 20, 21 and 22days of pregnancy. The rats were implanted with silicon tubes filled with pregesterone on day 15 of pregnancy. The progesterone and 20$\alpha$-OHP in serum were assayed by radioimmunoassay. The results obtained are summarized as follows : 1. The progesterone levels in rats of control group showed 126.36$\pm$20.19ng/ml on 18days and gradually decreased to 69.3$\pm$11.9, 29.2$\pm$1.8ng/ml 20 and 21 days. 2. The progesterone levels after progesterone-tube implantation showed 120.1$\pm$8.5, 59.01$\pm$3.1, 85.33$\pm$17.9 and 62.9$\pm$6.1ng/ml on 18, 20, 21 and 22days of pregnancy, respectively. 3. The 20$\alpha$-OHP levels in rats of control group showed 68.5$\pm$8.0ng/ml on 18 days and gradually increased to 139.9$\pm$3.7 and 141.4$\pm$6.7ng/ml on 21 and 22days. 4. The 20$\alpha$-OHP levles after progesterone-tube implantation showed 108.5$\pm$32.7 and 106.4$\pm$10.6ng/ml on 18 and 20days and increased to reach the peak level at 21days(225.01$\pm$9.9ng/ml) and rapidly decreased on 22days(85.9$\pm$10.7ng/ml).

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Studies on the Pregnancy Diagnosis from Monoclonal Antigen of Progesterone (Progesterone Monoclonal Antigen에 의한 임신진단에 관한 연구)

  • ;Ono Hitoshi
    • Korean Journal of Animal Reproduction
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    • v.11 no.2
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    • pp.132-138
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    • 1987
  • This study was carried out to evaluate the ability of clinical application of pregnancy diagnosis based upon the determinatin of progesterone in milk, utilizing a chymosin inhibitor labelled with progesterone and monoclonal antibody to progesterone, and its compared with progesterone concentrations in the milk were assayed by radioimmunoassay. 1. The progesterone concentration of the pregnant cows (2.07$\pm$0.54ng/ml) were significantly higher than those of non-pregnant cows (1.04$\pm$0.19 ng/ml), and thereafter began to increase and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from monoclonal antigen of progesterone were 92.9% for non-pregnant cows, and 88.5% for pregnant cows. 3. During 20 to 22 days after artificial inseminatin, the accuracy of pregnancy diagnosis from milk progesterone concentrations were 92.9% for non-pregnant cows(<3.4ng/ml), and 92.3% for pregnant cows( 4.0ng/ml). The average overall accuracy of pregnancy prediction for pregnant and non-pregnant cows were 92.6%. 4. Accordingly, the pregnancy diagnosis from monoclonal antigen of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate result.

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Characteristics and application of monoclonal antibody to progesterone II. Development of progesterone enzyme-linked immunosorbent assay(ELISA) (Progesterone의 단크론성 항체에 관한 특성 및 활용에 관한 연구 II. ELISA 기법의 개발)

  • Kang, Chung-boo;Kim, Jong-shu
    • Korean Journal of Veterinary Research
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    • v.31 no.4
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    • pp.403-409
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    • 1991
  • This experiment was carried out to develop a sensitive, rapid, solid-phase microtitre plate assay of progesterone using the monoclonal antibody to this hormone. Monoclonal antibody to progesterone was much higher titre and binding affinity about 10 times than conventional polyclonal antibody to progesterone. Dot-blot analysis of monoclonal antibody revealed a single precipitation band when reacted with anti-mouse IgM and anti-mouse K. A competitive reaction was used with a reaction time of 2 hours. The standard dose-response curve was linear through 1,000pg/well. This ELISA system approach is applicable to evaluation for the rapid assessment of luteal function and reproductive status in both clinical and research in a wide variety of species.

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Characteristics and application of monoclonal antibody to progesterone I. Production of monoclonal antibody to progesterone (Progesterone의 단크론성 항체에 관한 특성 및 활용에 관한 연구 I. 단크론성 항체의 생산)

  • Kang, Chung-boo;Kim, Yong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.511-513
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    • 1990
  • Monoclonal antibody to progesterone was produced using the antigen $11{\alpha}$-hydroxyprogesterone hemisuccinate conjugated to bovine serum albumin. Hybridomas secreting antibody to progesterone were detected by radioimmunoassay and enzyme-linked immunosorbent assay and cloned in soft agar. Two stable monoclonal antibodies which were highly specific to progesterone were obtained, so it may be advantageously used to study on several physiological functions of progesterone including immunological research.

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