Characteristics of an Entomopathogenic Fungus Infecting Corythucha ciliata (Hemiptera: Tingidae)

버즘나무방패벌레 기생성 곰팡이의 특성 구명

  • Koo, Chang-Duck (Department of Forest Science, Chungbuk National University) ;
  • Lee, Seung-Kyu (Department of Forest Disease and Insect, Korea Forest Research Institute) ;
  • Kim, Seong Hwan (Department of Microbiology, Dankook University)
  • 구창덕 (충북대학교 농업생명환경대학 산림학전공) ;
  • 이승규 (국립산림과학원 산림병해충과) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2006.11.23
  • Accepted : 2006.12.15
  • Published : 2007.03.31

Abstract

Overwintering adults of sycamore lace bug (Corythucha ciliata) infected by an unidentified pathogenic fungus were found on the stems of street trees of sycamore in Cheongju city. The objective of this study was to describe this entomopathogenic fungus infecting overwintering sycamore lace bug adults. This unidentified fungus colonized the insect adult body and formed white colony with subglobose clusters of conidiocarps. The size of conidiocarps was 300 to $400{\mu}m$ and each conidium was 15 to $20{\mu}m$. The conidiospore was globus and 2.5 to $3.0{\mu}m$ in diameter, and the hyphae were 1 to $5{\mu}m$ thick. This fungus was successfully isolated and cultivated on potato dextrose agar medium (PDA). The fungal colony was white and then became light yellow. When conidia from this pure culture were inoculated into the overwintering adults, the fungus formed conidiocarps with the same morphology on the insect body and the lethal rate by the fungus was $88{\pm}16%$. This fungus has over 99% homology with Cordyceps bassiana (imperfect fungal name is Beauveria bassiana) in ITS-5.8s rDNA base sequence. The fungal ecology and the infection process of the fungus into its host need to be clarified before using this fungus as a biological control agent.

월동 중인 버즘나무방패벌레 성충이 흰색의 분생포자로 덮혀 있음이 청주시내 가로수에서 확인되었다. 이 연구의 목적은 월동중인 방패벌레 성충에 감염하는 곰팡이에 대하여 특성을 구명하는 것이다. 이 균이 형성한 분생자과는 $300-400{\mu}m$, 각 분생자는 $15-20{\mu}m$, 그리고 각 분생포자는 $2.5-3.0{\mu}m$, 균사의 굵기는 $1-5{\mu}m$이었다. 이 균은 감자한천배지(PDA)에서 5-6일이면 성충몸체에서 분리배양되었고 흰색의 콜로니는 옅은 황색으로 변하였다. 순수배양된 이 균의 분생포자과에서 채집한 분생포자를 월동중인 버즘나무방패벌레의 비감염 성충에 접종하였을 때 같은 형태의 분생포자과를 형성하였으며, 성충의 치사율은 $88{\pm}16%$였다. 이 균의 ITS-5.8s rDNA 염기서열은 Cordyceps bassiana(불완전균 이름은 beauveria bassiana)의 염기서열과 99% 이상의 유사성이 있었다. 앞으로 이 균의 생태적 특성과 기주에 대한 감염과정 등을 구명하여 생물학적 방제제로서 이용가능성을 연구할 필요가 있다고 생각한다.

Keywords

Acknowledgement

Supported by : 충북대학교

References

  1. 김길하,최미현,김정화. 1999 버즘나무방패벌레(노린재목 방패벌레과) 발육과 생식에 미치는 온도의 영향. 한국응용곤충학회지 38(2): 117-121
  2. 박지두,김철수,이상길,박영석,염승호,신상철. 1999. 버즘나무 방패벌레의 발육에 미치는 온도의 영향. 한국임학회지 88: 555-561
  3. 우건석 외 9명. 2002. 곤충분류학. 집현사. pp. 483
  4. 이귀용 등. 2005. 초미립자분무기에 의한 버즘나무방패 벌레 방제. 충북산림환경연구소 연찬회 자료. pp. 108
  5. 이범영,정영진,1997. 한국수목해충. 성안당. pp. 459
  6. 정영진,권태성,여운홍,변봉규,박철하. 1996. 새로운 침입해충 버즘나무방패벌레 발생. 한국응용곤충학회지 35: 137-139
  7. Arnette,R. H. Jr. 1985. American insects. A Handbook of the insects of America North of Mexico. Van Nostrand Reinhold Co. New York. pp 850
  8. Benjamin,R.K. 외 11 명. 2004. Recommended protocols for sampling particular groups of fungi: collecting and isolation protocols for fungi associated with animals. Insect- and other arthropod-associated fungi. In Biodiversity of Fungi. Inventory and monitoring methods. edited by G.M. Mueller, G.F. Bills and M.S. Foster. pp 395-433
  9. Domsch, K. H. 1980. Compendium of soil fungi. Vol 1. Academic Press. pp 136-140
  10. Fuxa, J.R. 1998. Environmental manipulation for microbial control of insects. In Conservation biological control. edited by P. Barbosa. Academic Press. London. pp. 255-268
  11. Halbert, S.E. and Meeker, J.R. 1998. The sycamore lace bug, Corythucha ciliata(Say) (Hemiptera: Tingidae). Entomology Circular No. 387, May/June 1998. Fla. Dept. Agric. & Consumer Services, Division of Plant Industry. pp.2
  12. Lad, O.B. and Hunagel, L.M. 2005. Population dynamics of the sycamore lace bug, Corythucha ciliata in Hungary. Appl. Ecol. Envi. Res. 4: 135-150
  13. Lucas, R. Managing pests and diseases. Craig Potton, 2005. Nelson, New Zealand. 400p
  14. Sparks, B., Braman, K. and Balsdon, J. 2002. Control of lace bug on ornamental plants. Cooperative Extension Service/The University of Georgia. Bulletin 1102. pp. 2
  15. Sung, J.M. Lee, J.O., Humber, R.A., Sung, G.H. and Shrestha, B. 2006. Cordyceps bassians and production of stromata in vitro showing Beauveria anamorph in Korea. Mycobiology 34: 1-6 https://doi.org/10.4489/MYCO.2006.34.1.001
  16. Wainhouse, D. 2005. Ecological methods in forest pest management. Oxford University Press. Oxford. pp. 228