Influence of pH, Emulsifier Concentration, and Homogenization Condition on the Production of Stable Oil-in-Water Emulsion Droplets Coated with Fish Gelatin

  • Surh, Jeong-Hee (Department of Food and Nutrition, College of Health and Welfare, Kangwon National University)
  • Published : 2007.12.31

Abstract

An oil-in-water (O/W) emulsion [20 wt% com oil, 0.5-6.0 wt% fish gelatin (FG), pH 3.0] was produced by high pressure homogenization, and the influence of pH, protein concentration, and homogenization condition on the formation of FG-stabilized emulsions was assessed by measuring particle size distribution, electrical charge, creaming stability, microstructure, and free FG concentration in the emulsions. Optical microscopy indicated that there were some large droplets ($d>10\;{\mu}m$) in all FG-emulsions, nevertheless, the amount of large droplets tended to decrease with increasing FG concentration. More than 90% of FG was present free in the continuous phase of the emulsions. To facilitate droplet disruption and prevent droplet coalescence within the homogenizer, homogenization time was adjusted in O/W emulsions stabilized by 2.0 or 4.0 wt% FG. However, the increase in the number of pass rather promoted droplet coalescence. This study has shown that the FG may have some limited use as a protein emulsifier in O/W emulsions.

Keywords

References

  1. Dickinson E. Hydrocolloids at interfaces and the influence on the properties of dispersed systems. Food Hydrocolloid 17: 25-39 (2003) https://doi.org/10.1016/S0268-005X(01)00120-5
  2. McClements DJ. Protein-stabilized emulsions. Curr. Opin. Colloid In. 9: 305-313 (2004) https://doi.org/10.1016/j.cocis.2004.09.003
  3. McClements DJ. Food Emulsions: Principles, Practice, and Techniques. CRC Press, Boca Raton, FL, USA. pp. 53-339 (2004)
  4. Charlambous G, Doxastakis G. Food Emulsifiers: Chemistry, Technology, Functional Properties, and Applications. Elsevier, Amsterdam, Netherlands. pp. 1-550 (1989)
  5. Garti N. What can nature offer from an emulsifier point of view: Trends and progress? Colloid Surface A 152: 125-146 (1999) https://doi.org/10.1016/S0927-7757(98)00621-9
  6. Stauffer CE. Emulsifiers. Eagen Press, St. Paul, MN, USA. pp. 1- 102 (1999)
  7. Krog NJ, Sparso FV. Food Emulsifiers: Their chemical and physical properties. pp. 45-91. In: Food Emulsions. Friberg S, Larsson K, Sjoblom J (eds). Marcel Dekker, NY, USA (2004)
  8. Kim MJ, Ha JY, Yun YR, Noh JS, Song YB, Song YO. Extension of shelf life of kimchi by addition of encapsulated mustard oil. Food Sci. Biotechnol. 15: 884-888 (2006)
  9. Oh JK, Kim SJ, Imm JY. Antioxidative effect of crude anthocyanins in water-in-oil microemulsion system. Food Sci. Biotechnol. 15: 283-288 (2006)
  10. Oh CH, Kwon YK, Jang YM, Lee DS, Park J. Headspace analysis for residual hexane in vegetable oil. Food Sci. Biotechnol. 14: 456- 460 (2005)
  11. Ledward DA. Gelation of gelatin. pp. 171-201. In: Functional Properties of Food Macromolecules. Mitchell JR, Ledward DA (eds). Elsevier Applied Science, London, UK (1986)
  12. Damodaran S. Amino acids, peptides, and proteins. pp. 321-429. In: Food Chemistry. Fennema OR (ed). Marcel Dekker, New York, NY, USA (1996)
  13. Surh J, Gu YS, Decker EA, McClements DJ. Influence of environmental stresses on stability of o/w emulsions containing cationic droplets stabilized by SDS-fish gelatin membranes. J. Agr. Food Chem. 53: 4236-4244 (2005) https://doi.org/10.1021/jf047944i
  14. Lobo L. Coalescence during emulsification; 3. Effect of gelatin on rupture and coalescence. J. Colloid. Interf. Sci. 254: 165-174 (2002)
  15. Muller HJ, Hermel H. On the relation between the molecular-mass distribution of gelatin and its ability to stabilize emulsions. Colloid. Polym. Sci. 272: 433-439 (1994) https://doi.org/10.1007/BF00659456
  16. Dickinson E, Lopez G. Comparison of the emulsifying properties of fish gelatin and commercial milk proteins. J. Food Sci. 66: 118-123 (2001) https://doi.org/10.1111/j.1365-2621.2001.tb15592.x
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275 (1951)
  18. Onsaard E, Vittayanont M, Srigam S, McClements DJ. Properties and stability of oil-in-water emulsions stabilized by coconut skim milk proteins. J. Agr. Food Chem. 53: 5747-5753 (2005) https://doi.org/10.1021/jf050312r
  19. Surh J, Loren SW, McClement DJ. Ability of conventional and nutritionally-modified whey protein concentrates to stabilize oil-inwater emulsions. Food Res. Int. 39: 761-771 (2006) https://doi.org/10.1016/j.foodres.2006.01.007
  20. Surh J, Decker EA, McClements DJ. Influence of pH and pectin type on properties of sodium-caseinate stabilized O/W emulsions. Food Hydrocolloid 20: 607-618 (2006) https://doi.org/10.1016/j.foodhyd.2005.07.004
  21. Toledano O, Magdassi S. Emulsification and foaming properties of hydrophobically modified gelatin. J. Colloid. Interf. Sci. 200: 235- 240 (1998) https://doi.org/10.1006/jcis.1997.5376