Identification of Lactic Acid Bacteria Involved in Traditional Korean Rice Wine Fermentation

  • Seo, Dong-Ho (Department of Food Science and Biotechnology, and Institute of Life Science and Resources, KyungHee University) ;
  • Jung, Jong-Hyun (Department of Food Science and Biotechnology, and Institute of Life Science and Resources, KyungHee University) ;
  • Kim, Hyun-You (Department of Food Science and Biotechnology, and Institute of Life Science and Resources, KyungHee University) ;
  • Kim, Young-Rok (Department of Food Science and Biotechnology, and Institute of Life Science and Resources, KyungHee University) ;
  • Ha, Suk-Jin (Department of Food Science and Biotechnology, and Institute of Life Science and Resources, KyungHee University) ;
  • Kim, Young-Cheul (Department of Nutrition, University of Massachusetts) ;
  • Park, Cheon-Seok (Department of Food Science and Biotechnology, and Institute of Life Science and Resources, KyungHee University)
  • Published : 2007.12.31

Abstract

Changes in microflora, pH, reducing sugar content, lactic acid content, and ethanol content during Korean rice wine fermentation were investigated. Typical quality characteristics of Korean rice wine fermentation including pH, reducing sugar content, lactic acid content, and ethanol content were evaluated. While a fungus was not detected in our Korean rice wine mash, yeast was found to be present at fairly high quantities (1.44-4.76\;{$\times}\;10^8\;CFU/mL$) throughout the fermentation period. It is assumed that lactic acid bacteria (LAB) had effects on the variations of fragrance and flavor for traditional Korean rice wine. The main LAB during the Korean rice wine fermentation was determined and identified as a Gram-positive, straight rod-shaped cell. Genotypic identification of the isolated strain by amplification of its 16S rRNA sequence revealed that the isolated strain was most closely related to Lactobacillus plantarum (99%) strains without any other comparable Lactobacillus strains. Therefore, we designated the major LAB identified from traditional Korean rice wine fermentation as L. plantarum RW.

Keywords

References

  1. Lee JH, Yu TS. Identification and characteristics of lactic acid bacteria isolated from nuruk. Korean J. Biotechnol. Bioeng. 15: 359-365 (2000)
  2. Yu TS, Kim HS, Hong J, Ha HP, Kim TY, Yoon IW. Bibliographical study on microorganisms of nuruk (Until 1945). J. Korean Soc. Food Nutr. 25: 170-179 (1996)
  3. Yu TS, Kim J, Kim HS, Hyun JS, Ha HP, Park MG. Bibliographical study on microorganisms of traditional Korean nuruk (Since 1945). J. Korean Soc. Food Nutr. 27: 789-799 (1998)
  4. Lee MK, Lee SW, Yoon TH. Quality assessment of yakju brewed with conventional nuruk. J. Korean Soc. Food Nutr. 23: 78-89 (1994)
  5. Lee SS, Kim KS, Eom AH, Sung CK, Hong IP. Production of Korean traditional rice-wines made from cultures of the single fungal isolates under laboratory conditions. Korean J. Mycol. 30: 61-65 (2002) https://doi.org/10.4489/KJM.2002.30.1.061
  6. So MH. Characteristics of a modified nuruk made by inoculation of traditional nuruk microorganisms. Korean J. Food Nutr. 12: 219-225 (1999)
  7. Lee TS, Choi JY. Volatile flavor components in mash of takju prepared by using Aspergillus kawachii nuruk. Korean J. Food Sci. Technol. 37: 944-950 (2005)
  8. Hong Y, Park SO, Choi EH. Quality characteristics of Andong-soju type mashes produced by the co-culture of wild microorganisms isolated from nuruk and mashes for soju (Koream traditional distilled liquor). Food Sci. Biotechnol. 7: 236-241 (1998)
  9. Hong Y, Kim YB, Park SO, Choi EH. Microflora and physiochemical characteristics of nuruk and main mashes during fermentation of a traditional Andong-soju. Food Biotechnol. 6: 297-303 (1997)
  10. Kodama S, Yamamoto A, Matsunaga A, Matsui K, Nakagomi K, Hayakawa K. Behaviors of D-and L-lactic acids during the brewing process of sake (Japanese rice wine). J. Agr. Food Chem. 50: 767- 770 (2002) https://doi.org/10.1021/jf011098j
  11. Rhee SJ, Lee CY, Kim KK, Lee CH. Comparison of the traditional (samhaeju) and industrial (cheongju) rice wine brewing in Korea. Food Sci. Biotechnol. 12: 242-247 (2003)
  12. Kim SJ. Potential probiotic properties of lactic acid bacteria isolated from kimchi. Food Sci. Biotechnol. 14: 547-550 (2005)
  13. Yu HE, Lee DH, Lee JH, Choi SY, Lee JS. Quality characteristics and cardiovascular activities of Korean traditional wines and liquors. Food Sci. Biotechnol. 14: 772-777 (2005)
  14. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428 (1959) https://doi.org/10.1021/ac60147a030
  15. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Wade WG. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S $\gamma$RNA. Appl. Environ. Microb. 64: 795-799 (1998)
  16. Kim JM, Kim KH, Kim SY, Park YS, Seo MJ, Yoon SS. Isolation and characterization of antilisterial lactic acid bacteria from kimchi. Food Sci. Biotechnol. 14: 503-508 (2005)
  17. Han EH, Lee TS, Noh BS, Lee DS. Quality characteristics in mash of takju prepared by using different nuruk during fermentation. Korean J. Food Sci. Technol. 29: 555-562 (1997)
  18. Kim TH, Lee JY, Jung SH, Kim YM, Jo JS, Chung DK, Lee HJ, Kim HY. Identification and distribution of predominant lactic acid bacteria in kimchi, a Korean traditional fermented food. J. Microbiol. Biotechn. 12: 635-642 (2002)
  19. Koh SK, Lee JE, Kim HW, Kim SS, Park YK, Park YH, Park YH, Koh KH. Identification and deacidification of lactic acid bacteria in Korean red wine. Food Sci. Biotechnol. 13: 96-99 (2004)