Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지

Effect of Chitin Compost on Biological control of Fusarium wilt in Tomato Field

키틴퇴비를 이용한 토마토의 Fusarium 시들음병의 생물학적 제어

  • Jin, Rong-De (Division of Applied BioScience and Biotechnology, and Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Cho, Min-Young (Division of Applied BioScience and Biotechnology, and Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Sung-Jae (Division of Applied BioScience and Biotechnology, and Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Ryu, Ji-Yeon (Division of Applied BioScience and Biotechnology, and Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Chae, Dong-Hyeon (Soil Love Co., Ltd.) ;
  • Kim, Yong-Woong (Division of Applied BioScience and Biotechnology, and Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Kil-Yong (Division of Applied BioScience and Biotechnology, and Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University)
  • 김영덕 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 조민영 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 김성재 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 유지연 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 채동현 ((주) 흙사랑) ;
  • 김용웅 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 김길용 (전남대학교 농업생명과학대학 응용생물공학부)
  • Received : 2006.01.16
  • Accepted : 2006.01.30
  • Published : 2006.02.28
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Abstract

Biological control by chitinolytic microorganisms is being evaluated as management options for soilborne diseases. Forty kilograms of chitin compost (CTC) and control compost (CC) were amended on tomato plots ($15m{\times}0.5m$) 7 d before transplanting to evaluate enzymatic activities and the control of Fusarium wilt. Samples were taken on day 1, 3, 5, and 7, the day 1 corresponded to the 66 d after transplanting, the day on which the initial wilting symptoms occurred in plants of CC treated plots. The chitinase activity in soil of CTC was always higher compared to the control. Pathogenesis related (PR) protein (chitinase, ${\beta}$-1, 3-glucanase and peroxidase) activities in tomato roots in CC increased every day and showed marked differences compared to CTC. Wilting symptoms (96 d after transplanting) were reduced by 25% in CTC compared to the control. Protection of tomato plant may be correlated with the high levels of soil enzyme activities resulting from the chitin compost.

최근 들어서 생물학적 제어 방법의 하나로써 키틴분해 미생물을 이용한 제어 수단이 식물병제어에 일정한 효과가 있는 것으로 보고되고 있다. Fusarium 시들음병을 억제하기 위하여 40kg의 키틴퇴비를 면적이 $7.5m^2$ 인 토양에 정식 7일전 처리하였으며 토마토가 시들음병 증세를 보이기 시작하는 날(정식 후 66일)로부터 시작하여 4번에 걸쳐 시료를 채취하였다. 키틴퇴비 처리구(CTC)의 근권토양의 키틴효소와 ${\beta}$-1,3-glucan 효소 활성은 일반퇴비 처리구 (CC) 토양 보다 항상 높은 값을 나타냈다. 그러나 식물체 뿌리에서 측정된 chitinase, ${\beta}$-1,3-glucanase, peroxidase과 같은 병 관련 효소들은 CNC에서 실험기간동안 증가 추이를 보였다. 실험의 마지막 단계인 정식 후 96일째에는 CTC의 토마토는 CC 와 비교 할 때 25% 낮은 치사율을 나타냈다.

Keywords

References

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