Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Proteomic Analysis and the Antimetastatic Effect of N-(4methyl)phenyl-O-(4-methoxy) phenyl-thionocarbamate-Induced Apoptosis in Human Melanoma SK-MEL-28 cells

  • Choi Su-La (Clinical Biochemistry Lab, Department of Pharmacy, College of Pharmacy, Chungnam National University, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Choi Yun-Sil (Clinical Biochemistry Lab, Department of Pharmacy, College of Pharmacy, Chungnam National University, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim Young-Kwan (Clinical Biochemistry Lab, Department of Pharmacy, College of Pharmacy, Chungnam National University, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Sung Nack-Do (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Chungnam National University, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kho Chang-Won (Division of Life Sciences, Korea Research Institute of Bioscience and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Park Byong-Chul (Division of Life Sciences, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Eun-Mi (Division of Life Sciences, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Jung-Hyung (Division of Life Sciences, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Kyung-Mee (Angio Lab.) ;
  • Kim Min-Yung (Angio Lab.) ;
  • Myung Pyung-Keun (Clinical Biochemistry Lab, Department of Pharmacy, College of Pharmacy, Chungnam National University, Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Published : 2006.03.01
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Abstract

We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrixassisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-kB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific cyclin D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic agents against the SK-MEL-28 human melanoma cell line.

Keywords

References

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