Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Stimulation of Cell Growth by Erythropoietin in RAW264.7 Cells: Association with AP-1 Activation

  • Seong Seu-Run (College of Pharmacy, Chungbuk National University) ;
  • Lee Jae-Woong (College of Pharmacy, Chungbuk National University) ;
  • Lee Yong-Kyoung (College of Pharmacy, Chungbuk National University) ;
  • Kim Tae-Il (College of Pharmacy, Chungbuk National University) ;
  • Son Dong-Ju (College of Pharmacy, Chungbuk National University) ;
  • Moon Dong-Cheol (College of Pharmacy, Chungbuk National University) ;
  • Yun Young-Won (College of Veterninary Medicine, Chungbuk National University) ;
  • Yoon Do-Young (Korea Research Institute of Bioscience and Biotechnology) ;
  • Hong Jin-Tae (College of Pharmacy, Chungbuk National University)
  • Published : 2006.03.01
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Abstract

Erythropoietin (EPO), a hematopoietic factor, is required for normal erythrocyte developments, but it has been demonstrated to have many other functions, and its receptor is localized in other tissues. In the present study, we investigated whether EPO can promote other cell proliferation and possible molecular mechanisms. EPO restored the inhibition of the RAW264.7 and PC12 cell growth by fetal bovine serum (FBS) withdrawal in a dose dependent manner, but not that of other cell types tested. The restoring effect of EPO was completed when the RAW264.7 cells were cultured in the medium containing as low as 3% of FBS, and 10 U/mL EPO could replace FBS. The restoring effect of EPO in the RAW264.7 cells was associated with the increased of c-Fos and c-Jun expression as well as AP-1 activation. These data demonstrate that EPO can stimulate RAW264. 7 cell as well as PC12 cell growth even when the cells were cultured without FBS or in the presence of small amounts of FBS in the medium, and this stimulating effect is associated with the activation of AP-1 transcription factor.

Keywords

References

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