Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Quantitative Analysis of Inosine as an Index Component from Larva of Allomirina dichotoma

장수풍뎅이 유충으로부터 지표성분(Inosine)의 분리 및 정량분석

  • 이진희 (경희대학교 생명과학대학) ;
  • 송명종 (경희대학교 생명과학대학) ;
  • 양혜정 (경희대학교 생명과학대학) ;
  • 방면호 (강화군 특화작목연구소) ;
  • 정동준 (경희대학교 생명과학대학) ;
  • 김영채 (경희대학교 생명과학대학) ;
  • 김세영 (경희대학교 생명과학대학) ;
  • 노영덕 (경희대학교 생명과학대학) ;
  • 정성현 (경희대학교 약학대학) ;
  • 백남인 (경희대학교 생명과학대학)
  • Published : 2006.12.31
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Abstract

A repeated silica Rel column chromatography of n-BuOH fraction from the larva of Allomirina dichotoma afforded a purified nucleic acid, which was identified as inosine on the basis of the spectroscopic data including IR, MS and NMR. The HPLC analysis of the index component using silica based $NH_2$-column exhibited the contents in the hot air-dried and Far-IR-dried larvae of Allomirina dichotoma as 0.003% and 0.055%, respectively.

장수풍뎅이 유충의 MeOH추출물에서 얻어진 n-BuOH 분획으로부터 silica gel column chromatography를 반복하여 핵산성분을 분리하였으며, NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여 inosine으로 동정하였다. Inosine을 지표성분으로 하여 $NH_2$-column을 이용한 HPLC 분석방법을 확립하였고, 열풍건조한 시료와 원적외선 건조기로 건조한 장수풍뎅이 유충 시료 중의 inosin의 함량을 각각 0.003% 및 0.055%로 정량분석하였다.

Keywords

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