Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Ulva lactuca Extracts on Cytotoxicity of Cancer Cell Lines and Immune Stimulation

갈파래(Ulva lactuca) 추출분획의 암 세포주에 대한 세포독성 및 면역활성 효과

  • Jang, Min-Kyung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Kim, Nam-Young (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Kim, Mi-Hyang (Department of Food and Nutrition, College of Medical Life Science, Silla University) ;
  • Bae, Song-Ja (Department of Food and Nutrition, College of Medical Life Science, Silla University) ;
  • Jang, Jeong-Su (Central Research Institute, Binex Co., Ltd.) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 장민경 (신라대학교 의생명과학대학 제약공학과) ;
  • 김남영 (신라대학교 의생명과학대학 제약공학과) ;
  • 이동근 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과) ;
  • 하종명 (신라대학교 의생명과학대학 제약공학과) ;
  • 하배진 (신라대학교 의생명과학대학 제약공학과) ;
  • 김미향 (신라대학교 의생명과학대학 식품영양학과) ;
  • 배송자 (신라대학교 의생명과학대학 식품영양학과) ;
  • 장정수 (㈜바이넥스 기업부설연구소) ;
  • 이상현 (신라대학교 의생명과학대학 제약공학과)
  • Published : 2006.12.01
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Abstract

Extracted fractions of the green seaweed Ulva lactuca were studied to verify the cytotoxicity and immunostimulating activity. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with solvents such as hexane, ethyl ether, methanol, butanol and $H_2O$. The cytotoxic effects of U. lactuca fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2) and rat glioma cell line (C6) were investigated. Ethyl ether fraction showed the highest cytotoxicity against all four cell lines tested. In addition, $H_2O$ fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of U. lactuca fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of U. lactuca extract stimulated NO production with concentration dependant manner. These results suggest that U. lactuca may be a useful candidate for a natural cancer preventing and immune-stimulating agents.

본 연구는 갈파래(Ulva lactuca) 추출분획의 암세포주에 대한 세포독성 및 면역활성 효과를 조사하였다. 갈파래의 에탄올 추출물로부터 분획물의 제조는 hexane, ethyl ether, methanol, butanol, $H_2O$의 용매를 이용하여 행하였다. 인간 백혈암세포주(U937), 생쥐 신경아종세포주(NB41A3), 인체간암세포주(HepG2),큰지 신경교세포주(C6) 등에 대한 갈파래 분획물의 세포독성을 측정하였다. 갈파래의 Ethyl ether 층은 4종류의 세포 모두에서 가장 높은 세포독성을 나타냈다. 또한 수층 역시 비교적 높은 세포독성을 나타냈다. 4종류의 세포 모두에서 농도의존적 경향을 나타냈다. 갈파래 분획물의 큰쥐 대식세포주(RAW 264.7)에 대한 면역활성 효과도 조사하였다. 갈파래 추출물의 5가지 분획물 모두에서 농도의존적으로 NO 생산을 활성화시켰다. 이러한 결과들로 잔파래가 항암 덴 면역활성을 나타내는 천연 소재개발에 있어 유용한 후보가 될 수 있을 것으로 사료된다.

Keywords

References

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