Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Characterization of Osh3, an Oxysterol-binding Protein, in Filamentous Growth of Saccharomyces cerevisiae and Candida albicans

  • Hur, Hyang-Sook (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Ryu, Ji-Ho (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Kwang-Hoon (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Jin-Mi (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University)
  • Published : 2006.10.31
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Abstract

OSH3 is one of the seven yeast homologues of the oxysterol binding proteins (OSBPs) which have the major binding affinity to the oxysterols and function as regulator of cholesterol biosynthesis in mammals. Mutational analysis of OSH3 showed that OSH3 plays a regulatory role in the yeast-to-hyphal transition through its oxysterol-binding domain in Saccharomyces cerevisiae. The OSH3 gene was also identified in the pathogenic yeast Candida albicans. Deletion of OSH3 caused a defect in the filamentous growth, which is the major cause of the C. albicans pathogencity. The filamentation defect of the mutation in the MAPK-associated transcription factor, namely $cph1{\Delta}$ was suppressed by overexpression of OSH3. These findings suggest the regulatory roles of OSH3 in the yeast filamentous growth and the functional conservations of OSH3 in S. cerevisiae and C. albicans.

Keywords

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