International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Isolation and Characterization of Plutella xylostella Granulovirus Isolated in Korea

  • Wang Yong (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi Jae Young (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kang Joong Nam (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim Yang-Su (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi Heekyu (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Roh Jong Yul (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Li Ming Shun (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Im Dae Joon (National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Je Yeon Ho (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Published : 2005.12.01
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Abstract

We have first isolated and characterized a Plutella xylostella granulovirus ($P_xGV$) from dead larvae of P. xylostella in Korea. The granule of $P_xGV$ was ovoidal shape with an approximate measure of $350-400nm{\times}150-200nm$, and each granule contained one single rod-shape virion with a mean size of $150-180nm{\times}20-30nm$. The major granule protein, granulin, had a molecular weight of approximately 29kDa. Whereas the nucleotide sequence of the granulin gene was identical to that of previously reported $P_xGV$, nucleotide sequences of two of three putative p10 genes were slightly different from those of reported $P_xGV$. These results suggested that the $P_xGV$ isolated in this study was a novel isolate containing different genomic information.

Keywords

References

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