• Title/Summary/Keyword: Bculovirus

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Isolation and Characterization of Plutella xylostella Granulovirus Isolated in Korea

  • Wang Yong;Choi Jae Young;Kang Joong Nam;Kim Yang-Su;Choi Heekyu;Roh Jong Yul;Li Ming Shun;Jin Byung Rae;Im Dae Joon;Je Yeon Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.2
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    • pp.87-91
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    • 2005
  • We have first isolated and characterized a Plutella xylostella granulovirus ($P_xGV$) from dead larvae of P. xylostella in Korea. The granule of $P_xGV$ was ovoidal shape with an approximate measure of $350-400nm{\times}150-200nm$, and each granule contained one single rod-shape virion with a mean size of $150-180nm{\times}20-30nm$. The major granule protein, granulin, had a molecular weight of approximately 29kDa. Whereas the nucleotide sequence of the granulin gene was identical to that of previously reported $P_xGV$, nucleotide sequences of two of three putative p10 genes were slightly different from those of reported $P_xGV$. These results suggested that the $P_xGV$ isolated in this study was a novel isolate containing different genomic information.