International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Mode of Transmission of a Newly Discovered Microsporidian and Its Effect on Fecundity and Hatching in Silkworm, Bombyx mori L.

  • Bhat Shabir Ahmad (Silkworm Pathology Laboratory, Central Sericultural Research and Training Institute(CSR&TI)) ;
  • Nataraju B. (Silkworm Pathology Laboratory, Central Sericultural Research and Training Institute(CSR&TI))
  • Published : 2005.12.01
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Abstract

The mode of transmission, effect on fecundity, hatching and tissues specificity of a microsporidian $(Lb_{ms})$ recovered from Lamerin breed of the silkworm Bombyx mori L. was studied and compared with standard strain Nosema bombycis. Peroral inoculation of $Lb_{ms}$ or N. bombycis to zeroday of $4^{th}$ instar larvae of silkworm was the most suitable method for producing information on development of stage specific mortality, pupation and obtaining infected adults for transovarial transmission studies. It was observed that pupal mortality, the percentage of moths emerged and the percentage of moths infected were significantly high in N. bombycis infected batches as compared $(Lb_{ms})$ in all the three tested breeds of the silkworm. However no significant difference was observed in larval mortality. The fecundity and hatchability was not affected significantly in $(Lb_{ms})$ infected adults, however significant reduction in egg production, fecundity, hatchability and increased egg retention was observed in mother moths infected with N. bombycis. The $(Lb_{ms})$ is transmitted both horizontally and vertically at lower rate due to its low rate of proliferation. The trans ovarial transmission of $(Lb_{ms})$ to the $F_1$ progeny generation through eggs averaged only $61.33\pm5.10\%$ whereas N. bombycis was transmitted at $100\%$. The $(Lb_{ms})$ had low oral infectivity and low transovarial transmission in silkworm B. mori.

Keywords

References

  1. Ananthalakshmi, K. V. V., T. Fujiwara and R. K. Datta (1994) First report on the isolation of three micvrosporidians (Nosema spp.) from the silkworm, Bombyx mori L. In India. Indian J. Seri. 33, 146-148
  2. Anderson, R. M. and R. M. May (1992) Diseases of Humans. Dynamics and Control. Oxford University Press, New York
  3. Bansal, A. K., N. N. Saxena, R. M. Shukla, D. K. Roy, B. R. R. P. Sinha and S. S. Sinha (1997) A technique proposed for estimation of pebrine in grainages. Sericolgia 37, 11-14
  4. Brook, W. M. (1988) Etomogenous protozoa; in CRC Handbook of Natural Pesticides. Vol. (V) Microbial Insecticides, Part A. Etomogenous Protozoa and Fungi. Ignoffo, C. M (ed.), pp. 1-150, CRC Press, Boca Raton
  5. Canning, E. U. (1990) Phylum Microspora; in Handbook of protoctista. Margulis, L., J. O. Corliss, M. Melkonian and D. L. Chapman (eds.), pp53-72, Jones and Bartlett Publishers, Boston
  6. Datta, R. K. (1992) Improvement of silkworm races (Bombyx mori L.) in India. Sericolgia 24, 393- 415
  7. Fujiwara, T. (1980) Three microsporidians (Nosema spp.) from the silkworm Bombyx mori. J. Seric. Sci. Jpn 49, 229-236
  8. Han, M. S. and H. Watanabe (1988) Transovarial transmission of two microsoridia in the silkworm, Bombyx mori, and disease occurrence in the progeny population. J. Invertebr. Pathol. 51, 41-45 https://doi.org/10.1016/0022-2011(88)90086-9
  9. Kishore, S., M. Baig, B. Nataraju, M. Balavenkatasubbaiah, V. Sivaprasad, M. N. S. Iyengar and R. K. Datta (1994) Cross infectivity microsporidians isolated from wild lepidopterous insects to silkworm, Bombyx mori L. Indian J. Seric. 33, 126-130
  10. Lim, J. S., Y. K. Lee, S. Y. Cho and M. S. Han (1982) Characteristics of a new microsporidian S80 isolated from the silkworm, Bombyx mori in Korea. Res. Proj. Rep. Korean Sericult. Assoco., Seoul
  11. Nageswara, Rao, M. Muthulaskshmi, S. Kanginakudra and J. Nagraju (2004) Phylogenetic relationships of three microsporidian isolates from silkworm, Bombyx mori. J. Invertebrt. Pathol. 3, 87-95
  12. Pasteur, L. (1870) Etude sur la maladie des vers a soie. Tome 1, p. 322, Tome II, p. 327 , Gauthier-Villars, Paris
  13. Samson, M. V., P. C. Santha, R. N. Singh and T. O. Sasidharan (1999a) A new microsporidian infecting Bombyx mori L. Indian Silk 37, 10-12
  14. Samson, M. V., P. C. Santha, R. N. Singh and T. O. Sasidharan (1999b) Microsporidian spore isolated from Pieris sp. Indian Silk 38, 5-8
  15. Sato, R. and H. Watanabe (1980) Purification mature microsporodian spores by isodensity equilibrium centrifugation. J. Sericult. Sci. Jpn 49,512-516
  16. Shabir Ahmad Bhat and B. Nataraju (2004) Preliminary study on a microsporidian isolate occurring in the Lamerin breed of the silkworm Bombyx mori L. in India. Int. J. Indust. Entomol. 2, 265-267
  17. Sharma, S. D., K. Chanderasekham, B. Nataraju, M. Balavetkatasubbaiah, T. Selvakumar, V. Thigarajan and S. B. Dandin (2003) The cross infectivity between pathogens of silkworm, Bombyx mori L. and mulberry leaf roller, Diaphania pulverulentlis (Hampson). Sericolgia 43, 203-209
  18. Tanaka, S., T. Shimizu, M. Kobayashi and R. Ishihara (1972) a new microsporidian pathogenic to the silkworm. Bombyx mori. J. Seric. Sci. Jpn 41,89-95
  19. Wasson, K. and R. L. Peper (2000) Mammalian microsporidiosis. Vet. Pathol. 37. 113-128 https://doi.org/10.1354/vp.37-2-113
  20. Weiser, J. (1991) Biological control of vectors (Mannual for collecting, field determination and handling of biofactors for control of vectors). John Wiley and Sons, NY, pp. 189
  21. Wittner, M. and L. M. Weiss (1999) The Microsporidia and microsporidiosis. ASM, Washington DC, pp. 1-553