Abstract
The objective of this work was to study separation of short peptide (glycine-tyrosine) by using supported liquid membranes (SLMs) containing Aliquat as a cationic carrier, In the present investigation, the influence of pH of donor phase, concentrations of carrier and salt concentrations of acceptor phase on separation flux rate were investigated. Below pH 7.0 the flux rate was not affected by NaCl concentration or carrier concentration. However, the rate was increased significantly above pH 7.0. The rate with Hossain's SLM(H-SLM) containing $20\%$ Aliquat was about 3-fold higher with pH 9.0 at 0.25 M NaCl and 10-fold higher with pH 8.0 at 1.0 M NaCl than that with Duggan's SLM(D-SLM) containing $8\%$ Aliquat respectively. Furthermore, the rate with H-SLM was 10-fold higher at 1.0 M NaCl than the rate with 0.25 M NaCl, In conclusion, it would appear that the rate of separation was facilitated by using high salt concentrations together with high carrier concentrations above pH 7.0.
SLM을 이용한 short peptide의 분리를 목적으로 donor phase의 pH 및 acceptor phase의 염농도, membrane의 carrier 농도 차에 따른 peptide의 이동속도를 확인한 결과는 다음과 같다. 즉, pH 7.0 이하에서는 acceptor phase의 NaCl 농도나 carrier 농도에 상관없이 Gly-Tyr의 이동속도는 거의 영향을 끼치지 않았고, pH 7.0 이상에서는 이동속도가 급격히 증가함을 알 수 있었다. 그리고 Aliquat가 $8\%$ 함유된 D-SLM과 $20\%$ 함유된 H-SLM의 경우 Gly-Tyr의 이동속도는 H-SLM이 D-SLM보다 pH 9.0, 0.25 M NaCl 용액에서는 약 3배, pH 8.0, 1.0 M NaCl용액에서는 10배 정도 빠르게 이동하였다. 또한 H-SLM에서 acceptor phase의 NaCl 농도가 1.0 M 인 경우 0.25 M에 비해 10배 정도 이동속도가 빠른 것으로 나타났다. 따라서, short peptide인 Gly-Tyr의 SLM을 이용한 추출에서 donor phase의 pH 7.0 이상, carrier 농도가 높은 H-SLM 사용, acceptor phase의 염 농도가 높을수록 이동속도가 빠른 것으로 나타났다.
