DOI QR코드

DOI QR Code

Bioconversion of Straw into Improved Fodder: Fungal Flora Decomposing Rice Straw

  • Helal, G.A. (Batany Department, Faculty of Science, Zagazig University)
  • Published : 2005.09.30

Abstract

The fungal flora decomposing rice straw were investigated all over the soil of Sharkia Province, east of Nile Delta, Egypt, using the nylon net bag technique. Sixty-four straw-decomposing species belonging to 30 genera were isolated by the dilution plate method in ground rice straw-Czapek's agar medium at pH 6. The plates were incubated separately at $5^{\circ}C,\;25^{\circ}C\;and\;45^{\circ}C$, respectively. Twenty nine species belonging to 14 genera were isolated at $5^{\circ}C$. The most frequent genus was Penicillium (seven species), and the next frequent genera were Acremonium (three species), Fusarium (three species), Alternaria, Chaetomium, Cladosporium, Mucor, Stachybotrys (two species) and Rhizopus stolonifer. At $25^{\circ}C$, 47 species belonging to 24 genera were isolated. The most frequent genus was Aspergillus (nine species), and the next frequent genera were ranked by Penicillium (five species), Chaetomium (three species), Fusarium (three species). Each of Alternaria, Cladosporium, Mucor, Myrothecium and Trichoderma was represented by two species. At $45^{\circ}C$, 15 species belonging to seven genera were isolated. These were seven species of Aspergillus, two species of Chaetomium and two species of Emericella, while Humicola, Malbranchea, Rhizomucor and Talaromyces were represented by one species respectively. The total counts of fungi the genera, and species per gram of dry straw were significantly affected by incubation temperature and soil analysis (P < 0.05).

Keywords

References

  1. Abdel-Hafez, A. L I., Mazen, M. B. and Galal, A. A. 1990. Glycophilic and cellulose-decomposing fungi from soils of Sinai Peninsula, Egypt. Arab Gulf J Scient. Res. 8: 153-168
  2. Abdel-Hafez, S. I. I. 1982. Cellulose-decomposing fungi of desert soil in Saudi Arabia. Mycopathologia 78: 73-78 https://doi.org/10.1007/BF00442629
  3. Abdel-Hafez, S. I. I., Moubasher, A. H. and Abdel-Fattah, H. M. 1978. Cellulose-decomposing fungi of salt marshes in Egypt. Folia Microbiol. 23: 37-44 https://doi.org/10.1007/BF02876594
  4. Abdel-Hafez, S. I. I., and Abdel-Kader, M. I. A. 1980. Cellulose-decomposing fungi of barley grains in Egypt. Mycopathologia 68: 143-147
  5. Abdel-Kader, M. I. A., Abdel-Hafez, A. I. I. and Abdel-Hafez, S. I. I. 1983. Composition of the fungal flora of Syrian soils. II Cellulose-decomposing fungi. Mycopathologia 81: 167-171 https://doi.org/10.1007/BF00436822
  6. Abdel-Sater, M. A. and El-Said, A. H. M. 2001. Xylan-decomposing fungi and xylanolytic activity in agricultural and industrial wastes. Int. Biodet. Biodegrad. 47: 15-21 https://doi.org/10.1016/S0964-8305(00)00113-X
  7. Arai, T, Takaya, T, Ito, Y, Hayakawa, K., Tshima, S., Shibuya, C, Nomura, M., Yoshimi, N., Shibayama, C and Yasuda, Y. 1998. Bronchial asthma induced by rice. Int. Med. 37: 98-101 https://doi.org/10.2169/internalmedicine.37.98
  8. Banerjee, S., Archana, A. and Satyanarayana, T 1995. Xylanolytic activity and xylan utilization by thermophilic molds. Folia Microbiol. 40: 279-282 https://doi.org/10.1007/BF02814208
  9. Bisaria, V. S. and Ghose, T. K. 1981. Biodegradation of cellulosic materials: Substrates, microorganisms, enzymes and products. Enz. Microb. Technol. 3: 90-104 https://doi.org/10.1016/0141-0229(81)90066-1
  10. Booth, C. 1977. Fusarium. CMI, Kew, Surrey, England
  11. Caldwell, R. 1973. Observations on the fungal flora of decompos- ing beech litter in soil. Trans. Britch. Mycol. Soc. 4: 249-261
  12. Cooney, D. G and Emerson, R. 1964. Thermophilic fungi. WH. Freman and Company. San Francisco and London
  13. Coronel, L. .M., Joson, L. M. and Mesina, O. G 1991. Isolation and screening of thermophilic fungi for cellulose production. Philippine J. Sci. 120: 379-389
  14. Domsch, K. H., Gams, Wand Anderson, T. H. 1980. Compendium of soil fungi. Academic Press, London
  15. Duncan, D. B. 1955. Multiple range and multiple (F) test. Biometrics 11: 1-45 https://doi.org/10.2307/3001478
  16. Dytham, C. 1999. Choosing and using statistics: A biologist's guide. Blackwell Science Ltd., London, UK. p147
  17. Ellis, M.B. 1971. Dematiaceous hyphomycetes. CMI, Kew, Surrey, England
  18. El-Dohlob, S. M., Friend, J. and Sherief, A. A. 1985. Xylan decomposing fungi in Egyptian soil. Proceedings of the Egyptian soil. Proc. Egypt. Bot. Soc. (Ismallia Conference) 4: 477-487
  19. El-Kady, I. A., Abdel-Hafez, S. I. I. and Moubasher, M. H. 1981 Survey of cellulose-decomposing fungi of wheat straw in Egypt. Mycopathologia 76: 59-64 https://doi.org/10.1007/BF00761900
  20. El-Nawawy, A. S., 1972. Single-cell protein from Egyptian raw materials. Agr. Res. Rev. 50: 129-137
  21. Gams, W Hoekstra, E. S. and Aptroot, A. 1998. CBS course of mycology, 4th ed. Printed by Ponsen and Looyen BV, Wageningen, the Netherlands
  22. Harper, S. H. T. and Lynch, J. M. 1982a. The kinetics of straw decomposition in relation to its potential to produce the phytotoxin acetic acid. J. Soil Sci. 32: 627-637 https://doi.org/10.1111/j.1365-2389.1981.tb01735.x
  23. Helal, G A. 1993. Halotolerant and Halophilic fungi in salt marshes of Egyptian soil. Egypt. J. Appl. Sci. 8: 205-225
  24. Helal, G A.. 2005. Bioconversion of straw into improved fodder: Mycoprotein production and cellulolytic activity of rice straw decomposing fungi. Mycobiology 33: 90-96 https://doi.org/10.4489/MYCO.2005.33.2.090
  25. House, G. T. and Stinner, R. F. 1987. Decomposition of plant residues into tillage agroecosystems. Influence of litter on mesh size and soil arthropods. Pedobiologia 30: 351-360
  26. Hudson, H. J. 1972. Fungal saprophytism studies in biology No. 32. Edward Arnold, London
  27. Jackson, W L. 1962. Soil chemical analysis. Constable and Co. Ltd., London
  28. ohnson, L. F., Curl, E. A., Bond, J. H. and Fribourg, H. A. 1959. Methods for studying soil micoflora. Plant disease relationships, Burgess Pub. Co., Minneapolis
  29. Kitch, M. A. and Pitt, J. I. 1992. A laboratory guide to the commen Aspergillus species and their teleomorphs. CSIRO, Sydney
  30. Kubicek, C. P. and Harman, G E. 1998. Trichoderma and Gliocladium. Taylor and Francis Ltd, London and Bristol
  31. Maheshwari, R., Bharadwaj, G and Bhat, M. K. 2000. Thermophilic fungi: Their physiology and enzymes. Microb. Molecul. Biol. Rev. 64: 461-488 https://doi.org/10.1128/MMBR.64.3.461-488.2000
  32. Mazen, M. B., Moubasher, A. H. and Abdel-Hafez, A. I. I. 1980. Some ecological studies on Jordanian soil fungi. II Cellulosedecomposing fungi. Naturalia Monspeliensia, Serie Bot. Fasc. 40: 1-12
  33. Menzies, J. D. 1957. A dipper technique for serial dilution of soil for microbial analysis. Soil Sci. Soc. Am. Proc. 21: 660
  34. Moharram, A. M., Abdel-Hafez, S. I. I. and Abdel-Sater, M. A. 1995. Cellulolytic activity of fungi isolated from different substrates from the New Vally Governorate, Egypt. Abhath alYarmouk. Pure Sci. Eng. 4: 139-152
  35. Morais, M. H., Romas, A. C. and Oliveira, J. S. 1999. Culture of Lentinus edodes ('Shiitake'). Silva-Lusitana 7: 153-171
  36. Moubasher, A. H. 1963. Selective effects of fumigation with carbon disulphide on the soil fungus flora. Trans. Brit. Mycol. Soc. 46: 338-344 https://doi.org/10.1016/S0007-1536(63)80025-X
  37. Moubasher, A. H. 1993. Soil fungi in Qatar and other Arab Countries. The Centre for Scientific and Applied Research, Doha, Qatar
  38. Moubasher, A. H., Abdel-Hafez, S. I. I. and E1-Maghraby, O. M. O. 1985. Studies on soil mycoflora of Wadi Bir-E1-Ain, Eastern Desert, Egypt. Cryptogamie Mycologie 6: 129-143
  39. Moubasher, A. H., and Mazen, M. B. 1990. Competitive colonization of buried cellulose film by soil fungi in Egypt. Bulletin of the Faculty of Science, Assiut University 19:165-172
  40. Mouchacca, J. 1997. Thermophilic fungi: Biodiversity and taxonomic status. Cryptogamie, Mycol. 18: 19-69
  41. Mouchacca, J. 2000a. Thermotolerant fungi erroneously reported in applied research work as possessing thermophilic attributes. World J Microbiol. Biotech. 16: 869-880 https://doi.org/10.1023/A:1008979123304
  42. Mouchacca, J.. 2000b. Thermophilic fungi and applied research: a synopsis of name changes and synonymies. World J Microbiol. Biotech. 16: 881-888 https://doi.org/10.1023/A:1008970306466
  43. Piper, C. S. 1947. Soil and plant analysis. Adelaide Univ
  44. Pitt, J. I. 1979. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Academic Press, London and New York
  45. Pitt, J. I. 1986. Alaboratory guide to common Penicillium species.CSIRO, Sydney
  46. Rai, J. P., Sinha, A. and Govil, S. R. 2001. Litter decomposing mycoflora of rice straw. Crop. Res. 21: 335-340
  47. Raper, K. B. and Fennell, D. I. 1977. The genus Aspergillus. Robert E.K. Publish. Co., Huntington, New York
  48. Raper, K. B. and Thom,C. 1968. A manual of the Penicillium. Hafner Publish. Co., New York
  49. Richards, L. A. 1954. Diagnosis and improvement of saline and alkali soils. U.S. Dept. Agric., Handbook, No. 60
  50. Rifai, M. A. 1969. A revision on the genus Trichoderma. Mycological papers 116: 1-52
  51. Samar, S., Malik, R. K., Mangat, R., Singh, S. and Ram, M. 1999. Effect of rice straw burning on the efficacy of the herbicides in wheat (Triticum aestivum). Ind. J Agronomy 44: 361-366
  52. Satyanarayana, T., Jain, S. and Johri, B. N. 1988. Cellulases and xylanases of thermophilic moulds. Pp. 24-60. In: Agnihotri, V P., Sarbhoy, A. K. and Kumar, D. Eds. Perspectives in Mycology and Plant Pathology, Malhotra Publ. House, New Delhi
  53. Smith, N. R. and Dawson, V. T. 1944. The bacteriostatic action of rose Bengal in media used for the plate count of soil fungi. Soil Sci. 58: 467-471 https://doi.org/10.1097/00010694-194412000-00006
  54. Snedecor, G. W. and Cochran, W. G. 1982. Statistical methods. 6th edition. Blackwell Science Ltd., London, UK. pp 147
  55. Soytong, K. 1991. Isolation of soil fungi and screening for their cellulose degradation properties. Kaen Kaset Khon Kaen Agricultural J. 19: 218-225
  56. Srinivasan, V R. 1979. Production of single-cell protein from cellulose. Pp 132-137. In Bioconversion of organic residues for rural communities. Louisiana State Univ., Baton Rouge, Louisiana, USA
  57. Tengerdy, R. P. and Szakacs, G. 2003. Bioconversion of lignocellulose in solid substrate fermentation. Bioch. Eng. J 13: 169-179 https://doi.org/10.1016/S1369-703X(02)00129-8
  58. Torigoe, K., Hasegawa, S., Numata, O., Yazaki, S., Matsumaga, M., Boku, N., Hiura, M. and Ino, H. 2000. Influence of emissipn from rice straw burning on bronchial asthma in children. Pediatr. Int. 42: 143-150 https://doi.org/10.1046/j.1442-200x.2000.01196.x
  59. Watson, R. D. 1960. Soil washing improves the value of the soil dilution and the plate count method of estimating populations of soil fungi. Phytopathol. 50: 792-794
  60. Wise, H. D. and Schaefer, M. 1994. Decomposition of leaf litter in a mull beech forest: Comparison between canopy and herbaceous species. Pedobiologia 38: 269-288
  61. Yananobe, T., Mitsuishi, Y. and Takasaki, Y. 1994. Method for production of cellulolytic enzymes and method for saccharifications of cellulosic materials. Agency of Industrial Science Technology, Ministry of International Trade Industry United States - Patent. US, 4956291
  62. Zayed, G and Abdel-Motaal, H. 2005. Bio-active composts from rice strawenriched with rock phosphate and their effect on the phosphorus nutrition and microbial community in rhizosphere of cowpea. Biores. Technol. 96: 929-935 https://doi.org/10.1016/j.biortech.2004.08.002

Cited by

  1. Bioconversion of Straw into Improved Fodder: Preliminary Treatment of Rice Straw Using Mechanical, Chemical and/or Gamma Irradiation vol.34, pp.1, 2006, https://doi.org/10.4489/MYCO.2006.34.1.014
  2. fermented rice straw on growth, blood, and rumen fluid parameters in Barbados sheep vol.46, pp.1, 2018, https://doi.org/10.1080/09712119.2018.1480487