Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Candida albicans Chitin Synthase 1 Inhibitor from Streptomyces sp. A6705 and Its Characterization

  • KIM NA RAE (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • HWANG EUI IL (Bio Research Group, KT&G Central Research Institute) ;
  • YUN BONG SIK (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • LEE SANG HAN (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • MOON JAE SUN (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • LIM CHI HWAN (Department of Agricultural Chemistry, Graduate School, Chungnam National University) ;
  • LIM SE JIN (College of Pharmacy, Dongduk Women's University) ;
  • KIM SUNG UK (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2005.08.01
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Abstract

In the course of searching for potent chitin synthase 1 inhibitors from natural resources, Streptomyces sp. A6705 was found to exhibit potent inhibitory activity against the chitin synthase 1 from C. albicans (CaCHS1p). As a result, the inhibitor was isolated and identified using a series of chromatographies. Through chemical analyses with UV spectrophotometry, MS spectrometry, and various NMR techniques, the inhibitor was identified as N,N-bis(2-phenylethyl)urea. The compound exhibited strong inhibitory activity against the chitin synthase 1 from C. albicans with an $IC_{50}$ of 14 ${\mu}g$/ml, representing a similar inhibitory activity to that of the well-known chitin synthase inhibitor, polyoxin D ($IC_{50}$ 15 ${\mu}g$/ml). However, the compound showed no inhibitory activity against the chitin synthase 2 of Saccharomyces cerevisiae up to 280 ${\mu}g$/ml, which is structurally and functionally analogous to CaCHS 1 p. In addition, the compound exhibited weak antifungal activities against Cryptococcus neoformans and Rhizoctonis solani.

Keywords

References

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