Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characteristics of Peptide Assimilation by Helicobacter pylori: Evidence for Involvement of Cell Surface Peptidase

  • YUN SOON-KYU (Graduate School of Biotechnology & Department of Bioinformatics and Biotechnology, Korea University) ;
  • CHOI KYUNG-MIN (Graduate School of Biotechnology & Department of Bioinformatics and Biotechnology, Korea University) ;
  • UHM CHANG-SUB (Department of Anatomy, College of Medicine, Korea University) ;
  • PARK JEONG-KYU (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • HWANG SE-YOUNG (Graduate School of Biotechnology & Department of Bioinformatics and Biotechnology, Korea University)
  • Published : 2005.08.01
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Abstract

Peptide assimilation by Helicobacter pylori was investigated using L-phenylalanyl-3-thia-phenylalanine (PSP) as a detector peptide; the release of thiophenol upon enzymatic hydrolysis of PSP was spectrophotometrically detected with the aid of 5,5'-dithiobis[2-nitrobenzoic acid] (DTNB). By adding PSP to whole-cell suspension, thiophenol was produced progressively, resembling that found in Esherichia coli or Staphylococcus aureus. Interestingly, the rate of thiophenol production by H pylori in particular was markedly reduced when cells were pretreated with trypsin, indicating surface exhibition of peptidase. According to the competitive spectrophotometry using alanyl-peptides, H pylori did not appear to assimilate PSP through the peptide transport system. No discernible PSP assimilation could be ascertained in H pylori cells, unless provided with some additives necessary for peptidase activity, such as $Ni^{2+}\;or\;Mg^{2+}$ and an appropriate concentration of potassium or ammonium salts. These observations strongly suggest that, regardless of a presumptive peptide transport system, peptide assimilation of H. plori appears to be highly dependent upon milieu conditions, due to unique peptidase exhibition on the cell surface.

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