Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Effect of Bombyx mori, Antheraea Yamamai and Antheraea pernyi Silk Protein in Skin Fibroblast Cell Proliferation After Injury

피부세포 증식에 관여하는 실크단백질 탐색

  • Han, Sang-Mi (Dept. of Agricultural Biology, NIAST, RDA) ;
  • Lee, Kwang-Gill (Dept. of Agricultural Biology, NIAST, RDA) ;
  • Yeo, Joo-Hong (Dept. of Agricultural Biology, NIAST, RDA) ;
  • Kweon, Hae-Yong (Dept. of Agricultural Biology, NIAST, RDA) ;
  • Woo, Soon-Ok (Dept. of Agricultural Biology, NIAST, RDA) ;
  • Baek, Ha-Ju (Dept. of Wasterwater Analysis, KyongsangBuk-Do Govement Public Institute of Health & Environment) ;
  • Park, Kwan-Kyu (Dept. of Pathology, Catholic University of Daegu School of Medicine)
  • 한상미 (농업과학기술원 농업생물부) ;
  • 이광길 (농업과학기술원 농업생물부) ;
  • 여주홍 (농업과학기술원 농업생물부) ;
  • 권해용 (농업과학기술원 농업생물부) ;
  • 우순옥 (농업과학기술원 농업생물부) ;
  • 백하주 (경상북도보건환경연구원) ;
  • 박관규 (대구가톨릭대학교 병리학교실)
  • Published : 2004.12.01
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Abstract

We have studied the effect of silk proteins to the cell proliferation of human skin fibroblast cells (CCD-986sk) after injury. Silk proteins were extracted treatment with enzyme or NaOH solution from raw silk and culled-cocoon shell of Bombyx mori, Antheraea yamamai and A. pernyi. The cell proliferation after in vitro injury are increased in treatment by Bombyx mori (BM-1,2), Antheraea yamami (AY-1,2) and A. pernyi (AP-1,2). The silk protein fractions-treated cells exhibited proliferation in a dose dependent between $0.1\;{\mu}g/ml$ and $10\;{\mu}g/ml$. But, the macrophage, RAW 264. 7 cell viability was unaffected by the silk protein fractions by MTT assay. The molecular weights of the silk protein fractions were from 300-600 to 900-1500. These results results that the silk protein fractions may function through skin fibroblast proliferation.

가잠과 천잠 및 작잠의 실크 단백질 분획물로부터 피부섬유아세포에 대한 증식 효과를 알아본 결과 다음과 같은 결과를 얻을 수 있었다. 1. 가잠 및 천${\cdot}$작잠 단백질로부터 다양한 분자량대의 실크 단백질을 순수 분리 분획화 하여 사람 피부세포(human skin fibroblast; CCD-986sk)에 기계적 상처를 준 후 처리한 결과, 평균분자량(Mw)이 300-500 및 950-1500 분획군이 세포 증식에 뛰어난 효과가 있음을 확인${\cdot}$선발하였다. 2. 가잠의 경우 평균 분자량 590(BM-1), 1400(BM-2)에서, 천잠은 340(AY-1), 940(AY-2), 작잠에서는 300(AP-1), 1440(AP-2)에서 $10\;{\mu}g/ml$의 농도로 처리했을 경우 피부섬유 아세포의 증식이 무처리구에 비하여 40% 이상의 증식 효과를 갖았다. 또한 유효 농도 범위에서 대식세포인 RAW 264.7 세포에 대해서는 세포독성을 지니지 않았다. 3. 아미노산 조성에 있어 저분자량 AY-1 분획의 전아미노산 및 유리아미노산은 Tyr 함량이 매우 높게(25-48 g/100g)나타났으며, 작잠의 저분자 분획인 AP-1에서는 Lys 함량이 높게(5.2 g/100g) 나타남을 확인하였다. 4. 동일한 실크 단백질일 경우 저분자량 분획물에서 피부 세포 증식에 좀 더 효과적이였다.

Keywords

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