Expression Patterns of P21-Activated Kinase 2 by Sterne Spores on Human Macrophages

사람 대식세포에 스턴아포에 처리에 의한 P21-activated kinase 2 단백질의 발현양상

  • Published : 2004.12.01

Abstract

In order to elucidate the mechanism of infection on human macrophages, we peformed the 2-dimensional electrophoresis and the western blot analysis using the infected human macrophages with the spores of live and inactivated Sterne. We confirmed P21-activated kinase 2 protein which related to cell death(apoptosis) human macrophages at the early stage events. The inhibition of the P21-activated protein kinase 2 protein will be reduced apoptosis on infected human macrophages with Sterne spores.

Keywords

References

  1. Bollag, D. M., Rozychi, M. D., Edelstein, S. J., Protein methods, 2ed. Wiley-Liss, Inc., 605 Thire Avenue, NY. pp.195-228 (1996)
  2. Guidi-Rontani, C. The alveolar macrophage: the Trojan horse of Bacillus anthracis. Trends Microbiol. 10, 2002, pp.405-409
  3. Guidi-Rontani, C., M. Weber-Levy, E. Labruyere, and M. Mock. Germination of Bacillus anthracis spores within alveolar macrophage. Mol. Microbiol. 31, 1999, pp.917
  4. Jacobi, R., McCarthy, C. C., Koeppel, M. A.,Stringer, D. K. Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation. J. Biol. Chem. 278, 2003, pp.38675 - 38685
  5. Lowry, O. H., Rosebrough, N.J, Farr, A. L., Randall, R. J., Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 1951, pp.265-275