The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
권호 표지

Molecular Nuclear Cardiac Imaging

심장핵의학 분자영상학

  • Lee, Dong-Soo (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
  • Paeng, Jin-Chul (Department of Nuclear Medicine, Seoul National University College of Medicine)
  • 이동수 (서울대학교 의과대학 핵의학교실) ;
  • 팽진철 (서울대학교 의과대학 핵의학교실)
  • Published : 2004.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Molecular nuclear cardiac imaging has included Tc-99m Annexin imaging to visualize myocardial apoptosis, but is now usually associated with gene therapy and cell-based therapy. Cardiac gene therapy was not successful so far but cardiac reporter gene imaging was made possible using HSV-TK (herpes simplex virus thymidine kinase) and F-18 FHBG (fluoro-hydroxymethylbutyl guanine) or I-124 FIAU (fluoro-deoxyiodo-arabino-furanosyluracil). Gene delivery was performed by needic injection with or without catheter guidance. Tk expression did not last longer than 2 weeks in myocardium. Cell-based therapy of ischemic heart or failing heart looks promising, but biodistribution and differentiation of transplanted cells are not known. Reporter genes can be transfected to the stem/progenitor cells and cells containing these genes can be transplanted to the recipients using catheter-based purging or injection. Repeated imaging should be available and if promoter are varied to let express reporter transgenes, cellular (trans)differentiation can be studied. NIS (sodium iodide symporter) or D2R receptor genes are promising in this aspect.

Keywords

References

  1. Hofstra L, Liem IH, Dumont EA, Boersma HH, van Heerde WL, Doevendans PA, et al. Visualisation of cell death in vivo in patients with acute myocardial infarction. Lancet 2000;356:209-12. https://doi.org/10.1016/S0140-6736(00)02482-X
  2. Flotats A, Carrio I. Non-invasive in vivo imaging of myocardial apoptosis and necrosis. Eur J Nucl Med Mol Imaging 2003;30:615-30. https://doi.org/10.1007/s00259-003-1136-y
  3. Bengel FM, Ueberfuhr P, Ziegler SI, Nekolla S, Reichart B, Schwaiger M. Serial assessment of sympathetic reinnervation after orthotopic hearttransplantation. A longitudinal study using PET and C-11 hydroxyephedrine. Circulation 1999;99:1866-71.
  4. Isner JM. Myocardial gene therapy. Nature 2002;415:234-9. https://doi.org/10.1038/415234a
  5. Isner JM, Feldman LJ. Gene therapy for arterial disease. Lancet 1994;344:1653-4. https://doi.org/10.1016/S0140-6736(94)90454-5
  6. Losordo DW, Vale PR, Hendel RC, Milliken CE, Fortuin FD, Cummings N, et al. Phase 1/2 placebo-controlled, double-blind, dose-escalating trial of myocardialvascular endothelial growth factor 2 gene transfer by catheter delivery inpatients with chronic myocardial ischemia. Circulation 2002;105:2012-8. https://doi.org/10.1161/01.CIR.0000015982.70785.B7
  7. Byun J, Heard JM, Huh JE, Park SJ, Jung EA, Jeong JO, et al. Efficient expression of the vascular endothelial growth factor gene in vitro and in vivo, using an adeno-associated virus vector. J Mol Cell Cardiol 2001;33:295-305. https://doi.org/10.1006/jmcc.2000.1301
  8. Inubushi M, Wu JC, Gambhir SS, Sundaresan G, Satyamurthy N, Namavari M, et al. Positron-emission tomography reporter gene expression imaging in rat myocardium. Circulation 2003;107:326-32. https://doi.org/10.1161/01.CIR.0000044385.60972.AE
  9. Bengel FM, Anton M, Richter T, Simoes MV, Haubner R, et al. Noninvasive imaging of transgene expression by use of positron emission tomography in a pig model of myocardial gene transfer. Circulation 2003;108:2127-33. https://doi.org/10.1161/01.CIR.0000091401.26280.A0
  10. Kang HJ, Kim HS, Zhang SY, Park KW, Cho HJ, Koo BK, et al. Effects of intracoronary infusion of peripheral blood stem-cells mobilised with granulocyte-colony stimulating factor on left ventricular systolic function and restenosis after coronary stenting in myocardial infarction: the MAGIC cell randomised clinical trial. Lancet 2004;363:751-6. https://doi.org/10.1016/S0140-6736(04)15689-4
  11. Siminiak T, Kurpisz M. Myocardial replacement therapy. Circulation 2003;108:1167-71. https://doi.org/10.1161/01.CIR.0000086628.42652.8D
  12. Barbash IM, Chouraqui P, Baron J, Feinberg MS, Etzion S, Tessone A, et al. Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium: feasibility, cell migration, and body distribution. Circulation 2003;108:863-8. https://doi.org/10.1161/01.CIR.0000084828.50310.6A
  13. Aicher A, Brenner W, Zuhayra M, Badorff C, Massoudi S, Assmus B, et al. Assessment of the tissue distribution of transplanted human endothelial progenitor cells by radioactive labeling. Circulation 2003;107:2134-9. https://doi.org/10.1161/01.CIR.0000062649.63838.C9
  14. Hill JM, Dick AJ, Raman VK, Thompson RB, Yu ZX, Hinds KA, et al. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation 2003;108:1009-14. https://doi.org/10.1161/01.CIR.0000084537.66419.7A
  15. Kraitchman DL, Heldman AW, Atalar E, Amado LC, Martin BJ, Pittenger MF, et al. In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction. Circulation 2003;107:2290-3. https://doi.org/10.1161/01.CIR.0000070931.62772.4E
  16. Kim YH, Lee DS, Jang JH, Lee YJ, Chung J-K, Lee MC. Expression of sodium/iodide symporter transgene in neural stem cells. Korean J Nucl Med 2004;38:99-108.
  17. Zeiher AM. Peipheral blood progenitors for cardiac repair. Heart Failure Symposium. 654 J Am Coll Cardiol. 2004. 268 (Abstract)
  18. Paeng JC, Lee DS, Kang JH, Chung J-K, Lee MC. Development of a transgenic muse model for imaging of cellular differentiation into cardiomyocyte. J Am Coll Cardiol. 2004. 43 (Suppl) 24A