The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of Hair Essence (HHRHG0202-80) Containing Five Herbal Extracts on Hair Growth and the Prevention of Alopecia in vitro & vivo

5종의 한약 추출물이 함유된 헤어 에센스제품(HHRHG0202-80)이 in vitro 및 in vivo에서 육모 및 탈모 방지에 미치는 효과

  • Published : 2004.03.01
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Abstract

Backgrounds : Androgenetic alopecia is a relatively common disorder, but its precise mechanism is not elucidated. There are two commercial drugs approved by FDA. One(finasteride) has an inhibition activity of 5$\alpha$-reductase(type 2) and the other(minoxidil) has a vasodilation activity. Objectives : A verified herbal remedy for baldness is needed for medicinal treatment or preventing alopecia, which could be demonstrated by alopecia-related in vitro & in vivo tests Methods : On the basis of oriental pharmacognosy, we classified many herbal medicines into four groups (malnutrition, aging, alopecia and gray hair) according to its effect. The mitosis induction of hairy dermal papillae cell and the metabolic inhibition for type 2 $5{\alpha}-reductase$ were tested with five herbal extracts. Also, five herbal extracts were added to the normal essence formulation (HHRHG0202-80) in ranges of 0.1~0.3%, which was applied two-mouse models to validate each hair growing activity in vivo. Results : Stimulation of follicular papillae cell proliferation was observed in treatment of three herbal extracts (Glycyrrhizae radix:159.7%, Corni fructus : 144.7%, and Coicis semen : 136.6%) at a dose of $10\mu\textrm{g}/ml$. Three herbal extracts (Biotae semen. Glycyrrhizae radix and Coicis semen) showed inhibitory activity for $5{\alpha}-reductase$(type 2) at 93.18%, 73.36% and 47.6%, respectively at the same dose. We observed the enhancement of hair growth activity in C57b1/6 mouse and the inhibition of alopecia in AGA mouse after topical administration of the hair essence. Conclusions : Hair essence product, which contains five medicinal plants, would be used for the remedy for male pattern baldness (MPB) and the other alopecia diseases.

Keywords

References

  1. J Am Acad Dermatol. v.128 Diagnostic and predictive value of horizontal sections of scalp biopsy specimens in male pattern androgenetic alopecia Whiting, D.A.
  2. Am J Med. v.98 Androgenetic alopecia: an autosomal dominant disorder Bergfeld, W.F.
  3. Horm Res. v.54 no.5-6 The hair follicle: a paradoxical androgen target organ Randall, V.A.;Hibberts, N.A.;Thornton, M.J.;Hamada K;Merrick, A.E.;Kato S;Jenner, T.J.;De Oliveira I;Messenger, A.G.
  4. J Am Acad Dermatol. v.45 no.SUP. 3 Complexities of androgen action McPhaul, M.J.;Young M
  5. Science v.186 Steroid 5 alpha-reductase deficiency in man: an inherited form of male pseudohermaphroditism Imperato-McGinley J;Guerrero L;Gauatier T(et al.)
  6. 동의보감 허준
  7. 기난잡증 효험단방전서 장준정
  8. 신선기방 999 장연순;이서
  9. 피부병 중약 외용제제 송조우
  10. 중의 피부 미용 방제대전 장민경
  11. J Dermatol Sci v.31 The extract of Thujae occidentalis semen inhibited 5a-reductase and androchronogenetic alopecia of B6CBAF1/j hybrid mouse Wonseok Park(et al.)
  12. J Cell Sci. v.107 Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype Filsell W;Little, J.C.;Stones, A.J.;Granger, S.P.;Bayley, S.A.
  13. Proc Natl Acad Sci USA v.87 Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases Andersson S;Russell, D.W.
  14. J Dermatol Sci. v.25 KF19418, a new compound for hair growth promotion in vitro and in vivo mouse models Akio Shirai;Jun-ichi Ikeda;Sagano Kawashima;Tatsuya Tamaoki;Toshikazu Kamiya
  15. Clin Dermatol. v.6 no.4 The effect of testosterone, cyproterone acetate, and minoxidil on hair loss in the androchronogenetic alopecia mouse Matias, J.R.;Orentreich N
  16. Lipids v.27 no.7 5c,11c,14c-eicosatrienoic acid and 5c,11c,14c,17c-eicosatetraenoic acid of Biota orientalis seed oil affect lipid metabolism in the rat Ikeda I;Oka T;Koba K;Sugano M;Lie Ken Jie MS
  17. J Ethnopharmacol. v.38 no.2-3 Immunological aspects of Chinese medicinal plants as antiageing drugs Xiao, P.G.;Xing, S.T.;Wang, L.W.
  18. Res Commun Mol Pathol Pharmacol. v.97 no.3 Zeaxanthin dipalmitate from Lycium chinense has hepatoprotective activity Kim, H.P.;Kim, S.Y.;Lee, E.J.;Kim, Y.C.;Kim, Y.C.
  19. Biol Pharm Bull. v.25 no.3 Zeaxanthin dipalmitate from Lycium chinense fruit reduces experimentally induced hepatic fibrosis in rats Kim, H.P.;Lee, E.J;Kim, Y.C.;Kim J;Kim, H.K.;Park, J.H.;Kim, S.Y.;Kim, Y.C.
  20. Am J Pathol. v.151 no.6 Analysis of apoptosis during hair follicle regression (catagen) Lindner G;Botchkarev, V.A.;Botchkareva, N.V.;Ling G;van der Veen C;Paus R
  21. Acta Derm Venereol. v.78 Proanthocyanidins from grape seeds promote proliferation of mouse hair follicle cells in vitro and convert hair cycle In vivo Takahashi, T;Kamiya, T;Yokoo, Y
  22. Br J Dermatol. v.134 no.3 A comparison of the culture and growth of dermal papilla cells from hair follicles from non-balding and balding (androgenetic alopecia) scalp Randall, V.A.;Hibberts, N.A.;Hamada K
  23. J Clin Endocrinol Metab. v.79 no.3 The effect of finasteride, a 5 alpha-reductase inhibitor, on scalp skin testosterone and dihydrotestosterone concentrations in patients with male pattern baldness Dallob, A.L.;Sadick, N.S.;Unger W;Lipert S;Geissler, L.A.;Gregoire, S.L.;Nguyen, H.H.; Moore, E.C.;Tanaka, W.K.
  24. J Enzyme Inhib. v.16 Stable expression of the human 5alpha-reductase isoenzymes type I and type II in HEK293 cells to identify dual and selective inhibitors Reichert W;Hartmann, R.W.;Jose J
  25. J Steroid Biochem Mol Biol. v.82 no.2-3 Inhibition of type 1 and type 2 5alpha-reductase activity by free fatty acids, active ingredients of Permixon Raynaud, J.P.;Cousse H;Martin, P.M.
  26. Urol Res. v.26 no.4 Cactus flower extracts may prove beneficial in benign prostatic hyperplasia due to inhibition of 5alpha reductase activity, aromatase activity and lipid peroxidation Jonas A;Rosenblat G;Krapf D;Bitterman W;Neeman I
  27. Int Urol Nephrol. v.28 no.3 In vitro inhibition of testicular delta 5-3 beta-hydroxysteroid dehydrogenase and prostatic 5 alpha-reductase activities in rats and humans by strogen forte extract Toth I;Szecsi M;Julesz J;Faredin I;Behnke B
  28. Biochem J. v.285 no.Pt 2 Inhibition of steroid 5 alpha-reductase by specific aliphatic unsaturated fatty acids Liang T;Liao S
  29. Biomaterials v.23 Promoted growth of murine hair follicles through controlled release of vascular endothelial growth factor Makoto Ozeki;Yasuhiko Tabata