Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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The Effect of agrobacterium Density on Transformation Efficiency in Apple

Agrobacterium 농도가 사과 형질전화 효율에 미치는 영향

  • Seong, Eun-Soo (National Horticultural Research Institute, RDA) ;
  • Cha, Ji-Eun (National Horticultural Research Institute, RDA) ;
  • Kim, Jeong-Hee (National Horticultural Research Institute, RDA) ;
  • Park, Seong-Whan (Faculty of National Resources and Life Science, Dong-A University) ;
  • Yu, Chang-Yeon (College of Agriculture and Life Sciences, Kangwon National University) ;
  • Song, Kwan-Jeong (Faculty of Horticultural Life Sciences, Cheju National University)
  • 성은수 (농촌진흥청 원예연구소) ;
  • 차지은 (농촌진흥청 원예연구소) ;
  • 김정희 (농촌진흥청 원예연구소) ;
  • 박성환 (동아대학교 생명자원과학부) ;
  • 유창연 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 송관정 (제주대학교 원예생명과학부)
  • Published : 2003.09.01
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Abstract

This study was conducted to find optimum bacterial density for improving the efficiency of transformation mediated by Agrobacterium tumefaciens in apples. Regeneration(15％) and transformation frequency(10％) were increased in resuspension-culture density $A_{600}$ 1.3 from preculture density $A_{600}$ 0.7 of Agrobacterium tumefaciens in ′Fuji′. In ′Gala′, 20% regeneration and 16% transformation frequency were observed at optimum bacterial density $A_{600}$ 0.7 form preculture density $A_{600}$ 1.3. ′Mclntosh as well as "Gala" were 25％regeneration and 10％ transformation frequency. Hence a frequency optimum condition of bacterial density for the efficient transformation of apple could be depend on apple genotypes.

Keywords

References

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