• Magazine of the Korean Society of Agricultural Engineers
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• v.32 no.E
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• pp.20-32
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• 1990

Abstract This study was conducted to pursue the normalization of frequency distribution by making an approach to the coefficient of skewness to nearly zero through the Box-Cox transformation, to get probable flood flows can be calculated by means of the transformation equation which has been derivated by Box-Cox transformation in the annual maximum series of the applied watersheds. It has been concluded that Box-Cox transfromation is proved to be more efficient than logarithmic, square root and SMEMAX transformation which is based on the trigonometric solution of a right triangle whose three verteces repesent the smallest, median and largest observed values of a population in making the coefficient of skewness nearer to zero. Consequently it is shown that probable flood flows according to the return period based on Box-Cox transformation are closer to the observed data as compared to other methods including SMEMAX transformation and fitted probability distributions such as the three parameter lognormal and the type I extremal distribution for the applied watersheds.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.215-219
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• 2003

This study was conducted to find optimum bacterial density for improving the efficiency of transformation mediated by Agrobacterium tumefaciens in apples. Regeneration(15％) and transformation frequency(10％) were increased in resuspension-culture density $A_{600}$ 1.3 from preculture density $A_{600}$ 0.7 of Agrobacterium tumefaciens in ′Fuji′. In ′Gala′, 20% regeneration and 16% transformation frequency were observed at optimum bacterial density $A_{600}$ 0.7 form preculture density $A_{600}$ 1.3. ′Mclntosh as well as "Gala" were 25％regeneration and 10％ transformation frequency. Hence a frequency optimum condition of bacterial density for the efficient transformation of apple could be depend on apple genotypes.

• Phonetics and Speech Sciences
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• v.9 no.1
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• pp.41-47
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• 2017

The main advantage of the statistical parametric speech synthesis is its flexibility in changing voice characteristics. A personalized text-to-speech(TTS) system can be implemented by combining a speech synthesis system and a voice transformation system, and it is widely used in many application areas. It is known that the fundamental frequency and the spectral envelope of speech signal can be independently modified to convert the voice characteristics. Also it is important to maintain naturalness of the transformed speech. In this paper, a speech synthesis system based on Hidden Markov Model(HMM-based speech synthesis, HTS) using the STRAIGHT vocoder is constructed and voice transformation is conducted by modifying the fundamental frequency and spectral envelope. The fundamental frequency is transformed in a scaling method, and the spectral envelope is transformed through frequency warping method to control the speaker's vocal tract length. In particular, this study proposes a voice transformation method using the correlation between fundamental frequency and vocal tract length. Subjective evaluations were conducted to assess preference and mean opinion scores(MOS) for naturalness of synthetic speech. Experimental results showed that the proposed voice transformation method achieved higher preference than baseline systems while maintaining the naturalness of the speech quality.

• Microbiology and Biotechnology Letters
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• v.11 no.3
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• pp.163-168
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• 1983

The penicillin resistant plasmid DNA was prepared from Streptomyces bobili YS-40, producing penicillinase, by the phenol extraction method and introduced into Bocillus subtilis IAM 12118 by the transformation procedure of Mahler method. The optimal pH and temperature on the transformation was 7.0, 3$0^{\circ}C$ respectively. Above 20 minutes contact of plasmid DNA and recipient cell was shown the high transformation frequency. The transformant of penicillin resistance was proportionally increased as increase of the DNA concentration. The addition of lysine in transformation system increased the transformation frequency about 6-fold and the addition of the chloramphenicol did not affect the transformation frequency.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.40-49
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• 1984

To investigate the factors influencing the artifical transformation in Escherichia coli, E. coli C600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCl-Etbr equilibrium density gradient centrifugation from E.coli HB 101. The influencing factors in the transformation such as concentration of calcium chloride, time of ice incubation, temperature and time of heat shock, time of gene expression, effects of plasmid DNA concentration and adding time were examined in these experiments. The results obtained were as follows; 1. The highest transformation frequency was observed in the treatments of 100 mM $CaCl_2$ before heat shock and the treatment of $CaCl_2$ was essential step in the process of E. coli transformation. 2. The highest transformation frequency was observed in the treatment of heat shock at $42^{\circ}C$ for 4 min. or $37^{\circ}C$ for 6 min., but the prolonged heat shock resulted a decreased transformation frequency. 3. Treatments of ice incubation at $0^{\circ}C$ for 45 min. before heat stocks or at $0^{\circ}C$ for 30min. after heat shock resulted an increased transformation frequency. 4. There was a linear relationship between DNA concentration and transformation frequency at the concentration of $8{\times}10^3$ recipient cells. The highest transformation frequency reached in carte of 7 mcg of donor DNA, but above 1 mcg of DNA concentration, transformation frequency was not remarkably increased. Addition of donor DNA just after the treatment of $CaCl_2$ was the best. 5. The best condition of gene expression at $37^{\circ}C$ were 40min. for TC-resistant gene and 100min. for AP-resistant gene. TC-resistant gene was higher in the transformation frequency and faster in the gene expression time than AP-resistant gene. In these results, the best conditions for the transformation of E. coli C 600 with pBR322 DNA were: treatment with 100mM $CaCl_2$, ice incubation at $0^{\circ}C$ for 45 min, heat shock at $42^{\circ}C$ for 4 min., 30 min. of ice incubation and incubation at $37^{\circ}C$ for 100min. for gene expression in that order.

• Journal of Mechanical Science and Technology
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• v.15 no.2
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• pp.232-238
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• 2001

Fast Fourier Transformation is employed to convert the head variation of a pipeline in the time domain to the amplitude of the frequency domain. Applying method of characteristics to a pipeline provides a significant frequency range for a surge introduced from the valve modulation. Inverse Fast Fourier Transformation and a Finite Impulse Response Filter can be used to remove any possible noise existing from the significant frequency range of an unsteady condition. A filtered signal shows higher potential for the inverse calculation of leakage detection than the noise-added signal does. The respective performances of Inverse Fast Fourier Transformation and a Finite Impulse Response Filter are compared in terms of leakage detection capability. Characteristics of the frequency range for multiple leakages were investigated to validate the effectiveness of the noise control method in the frequency domain.

• Journal of KSNVE
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• v.10 no.5
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• pp.800-805
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• 2000

To determine the modal matrix and modal frequency of engine mount system, we most solve so-called eigen-value problem. However eigen-value problem of engine mount system with hydraulic mount can not be solved by general eigne-analysis algorithm because the properties of hydraulic mount vary with frequency. so in this paper the method for modal analysis of rigid body motions of an engine supported by hydraulic mount is proposed. Natural frequencies and mode shapes of this nonlinear system are obtained by using complex exponential method and Laplace transformation method. In time domain, impulse response functions are calculated by (two-sided) discrete inverse Fourier Transformation of forced frequency response functions achieved by Laplace transformation of the differential equation of motion. Considering the fact that frequency response functions synthesized by modal parameters form proposed method are in good agreement with original FRFs, it is proved that the proposed method is very efficient and useful for the analysis of eigne-value problem of hydraulic engine mount system.

• Journal of the Optical Society of Korea
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• v.14 no.2
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• pp.104-108
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• 2010

We proposed an axis transformation technique which reveals a distance parameter directly from optical scanning holography (OSH). After synthesis of a real-only spectrum hologram and power fringe adjusted filtering, we transform an original frequency axis to a new frequency axis using interpolation. In the new frequency axis, the filtered hologram has a single frequency which is linearly proportional to the distance parameter. Thus, the inverse Fourier transformation of the filtered hologram gives a delta function pair in the new spatial axis. Finally, we extract the distance parameter by detecting the location of the delta function pair.

• Proceedings of the IEEK Conference
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• 2003.11b
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• pp.255-258
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• 2003

This paper defines Normalized Term Frequency Weighting method for automatic text categorization by using Box-Cox, and then it applies automatic text categorization. Box-Cox transformation is statistical transformation method which makes normalized data. This paper applies that and suggests new term frequency weighting method. Because Normalized Term Frequency is different from every term compared by existing term frequency weighting method, it is general method more than fixed weighting method such as log or root. Normalized term frequency weighting method's reasonability has been proved though experiments, used 8000 newspapers divided in 4 groups, which resulted high categorization correctness in all cases.

• The Korean Journal of Food And Nutrition
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• v.5 no.2
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• pp.77-83
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• 1992

The optimum conditions and mechanisms for the plasmid-mediated genetic transformation of intact cells of Bacillus brevis Pl76-2, an extracellular protein producing bacterium by electroporation were investigated. It was found that pUB110 Plasmid DNA can be introduced into intact bacterial cells by electroporation. The frequency of transformation by this electroporation system depended upon the initial electric field strength, the capacity of the electric discharge capacitor, growth stage, number of successive pulses and composition of electroporation buffer. It was effective for transformation that cells were harvested, washed and resuspended with HSM [7M HEPES(PH 7.4), 272mM sucrose, 1 mM MgCl2] electroporation buffer when cell growth was attained to 1.2 at OD660. A maximum frequency of transformation of 2.40$\times$104 transformants per$\mu$g plasmid DNA was obtained by two succesive Pulses with an initial electric field strength of 12.5kV/cm and with a capacitance of 7.3uF.