Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Analysis of Genetic Variation of Perilla Germplasm Using RAPD

RAPD를 이용한 들깨 유전자원의 유전적 변이 분석

  • Kim, Doh-Hoon (College of Natural Resources and Life Science, Dong-A University) ;
  • Yang, Bo-Kyung (College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Hyeon-Kyoung (College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Na-Young (College of Natural Resources and Life Science, Dong-A University) ;
  • Jeong, Soon-Jae (College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Ik-Soo (Department of Sericulture and Entomology, NIAST, RDA) ;
  • Nam, Jae-Sung (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Jai-Heon (College of Natural Resources and Life Science, Dong-A University) ;
  • Chung, Dae-Soo (College of Natural Resources and Life Science, Dong-A University)
  • 김도훈 (동아대학교 생명자원과학대학) ;
  • 양보경 (동아대학교 생명자원과학대학) ;
  • 김현경 (동아대학교 생명자원과학대학) ;
  • 김나영 (동아대학교 생명자원과학대학) ;
  • 정순재 (동아대학교 생명자원과학대학) ;
  • 김익수 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 남재성 (동아대학교 생명자원과학대학) ;
  • 이재헌 (동아대학교 생명자원과학대학) ;
  • 정대수 (동아대학교 생명자원과학대학)
  • Published : 2003.09.01
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Abstract

Genetic variation of Perilla germplasms was investigated using RAPD markers. Forty-two Perilla frutescens lines and cultivars collected form locals were subjected to RAPD analysis using 220 primers. Among them only 13 primers showed polymorphic bands and these 13 primers provided a total of 144 bands, consist of 115 polymorphic and 29 monomorphic ones. The polymorphic bands were subjected to phylogenetic analysis using UPGMA and maximum parsimony (MP) methods. In the UPGMA method, similarity coefficiency of 42 Perilla frutescens lines and cultivars ranged from 0 to 0.7842. The dendrogram of 42 lines and cultivars obtained through UPGMA method resulted in two major groups, and the similar clustering pattern was found by MP method, suggesting Perilla germplasms utilized in this study truly can be divided into two major groups. Although the two major groups were consistent roughly with their phenotypes (under of node, weight of 1,000 grains, and oil content), in detail, much inconsistency also was present.

Keywords

References

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