Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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The Detection and a Quantitative Evaluation of Entomopathogenic Nematodes in Cultivated Rhizosphere Soil

경작지 근권 토양내 곤충병원성 선충의 검출 및 정량적 평가

  • 황경숙 (목원대학교 생명과학부 미생물학과) ;
  • 한상미 (농촌진흥청 농업과학기술원) ;
  • 김윤지 (목원대학교 생명과학부 미생물학과) ;
  • 남필원 (목원대학교 생명과학부 미생물학과) ;
  • 한송이 (목원대학교 생명과학부 미생물학과)
  • Published : 2003.09.01
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Abstract

The direct count and MPN (Most Probable Number) methods were used to measure the number of nematodes in soils collected from cultivated and non-cultivated fields. As a result, the number of nematodes from cultivated soils was higher than the non-cultivated soils (NC -1, NC -2). On the other hand, upon measuring the value from the organo farming cultivated soils (OC, OR) and conventional cultivated soils (CC, CR), the former showed 16 times higher than the latter. These results indicate that nematode population which can multiply in the organic compounds abundantly exist in the organo farming cultivated soil. Isolated entomopathogenie nematodes are composed of two orders, which were Rhabditida and Diplogasterida. To determine the pathogene-city of them using the 5th larvae and pupae silkworm, and the following mean $LD_{90}$ values were found: 24 to 30 hours in Rhabditida and 36 to 48 hours in Diplogasterida nematode, respectively. This study indicates that nematodes are sensitive to this kind of environmental disturbance. Isolated entomopathogenic nematodes were suggested that aye quite within the realms of possibility for biological control agents.

유기농업을 수행해온 배추와 무의 근권토양과 관행농법의 근권토양 및 비경작지 토양으로부터 토양시료를 채취하여 선충의 밀도를 조사하고, 토양내 유익선충인 곤충병원성 선충의 분포상황을 검토하였다. 경작지 토양 내 선충 분포율이 비경작지에 비하여 높았고, 유기농업 토양이 관행농업 토양보다 선충의 밀도가 높게 나타났다. 선충의 정량적 평가법으로 직접계수법과 MPN 법을 비교 조사한 결과 MPN법이 신속하고 정확한 방법임이 확인되었다. 각 토양으로부터 분리된 곤충병원성 선충은 Rhabditida목 선충과 Diplogasterida목 선충으로 분류되었다. 이들 선충 중 Diplogasterida목 선충이 50％ 이상을 차지하여 높은 점유율을 나타내었다. 누에를 기주체로 사용한 살충력 검정에 있어 24∼36시간 이내에 90％이상의 기주 치사율을 보여 농작물 해충방제를 위한 생물농약으로의 활용 가능성을 나타내었다.

Keywords

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