• Title/Summary/Keyword: entomophopathogenic nematode

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The Detection and a Quantitative Evaluation of Entomopathogenic Nematodes in Cultivated Rhizosphere Soil (경작지 근권 토양내 곤충병원성 선충의 검출 및 정량적 평가)

  • 황경숙;한상미;김윤지;남필원;한송이
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.271-275
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    • 2003
  • The direct count and MPN (Most Probable Number) methods were used to measure the number of nematodes in soils collected from cultivated and non-cultivated fields. As a result, the number of nematodes from cultivated soils was higher than the non-cultivated soils (NC -1, NC -2). On the other hand, upon measuring the value from the organo farming cultivated soils (OC, OR) and conventional cultivated soils (CC, CR), the former showed 16 times higher than the latter. These results indicate that nematode population which can multiply in the organic compounds abundantly exist in the organo farming cultivated soil. Isolated entomopathogenie nematodes are composed of two orders, which were Rhabditida and Diplogasterida. To determine the pathogene-city of them using the 5th larvae and pupae silkworm, and the following mean $LD_{90}$ values were found: 24 to 30 hours in Rhabditida and 36 to 48 hours in Diplogasterida nematode, respectively. This study indicates that nematodes are sensitive to this kind of environmental disturbance. Isolated entomopathogenic nematodes were suggested that aye quite within the realms of possibility for biological control agents.