• Title/Summary/Keyword: Diplogasterida

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The Detection and a Quantitative Evaluation of Entomopathogenic Nematodes in Cultivated Rhizosphere Soil (경작지 근권 토양내 곤충병원성 선충의 검출 및 정량적 평가)

  • 황경숙;한상미;김윤지;남필원;한송이
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.271-275
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    • 2003
  • The direct count and MPN (Most Probable Number) methods were used to measure the number of nematodes in soils collected from cultivated and non-cultivated fields. As a result, the number of nematodes from cultivated soils was higher than the non-cultivated soils (NC -1, NC -2). On the other hand, upon measuring the value from the organo farming cultivated soils (OC, OR) and conventional cultivated soils (CC, CR), the former showed 16 times higher than the latter. These results indicate that nematode population which can multiply in the organic compounds abundantly exist in the organo farming cultivated soil. Isolated entomopathogenie nematodes are composed of two orders, which were Rhabditida and Diplogasterida. To determine the pathogene-city of them using the 5th larvae and pupae silkworm, and the following mean $LD_{90}$ values were found: 24 to 30 hours in Rhabditida and 36 to 48 hours in Diplogasterida nematode, respectively. This study indicates that nematodes are sensitive to this kind of environmental disturbance. Isolated entomopathogenic nematodes were suggested that aye quite within the realms of possibility for biological control agents.

Screening of Tissue Papers for Nematode Extraction for the Baermann Funnel Method (Baermann Funnel법의 선충 분리 여과지 선발)

  • Kang, Heonil;Eun, Geun;Ha, Jihye;Lee, Jaehyun;Kim, Donggeun;Kim, Yongchul;Choi, Insoo
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.377-381
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    • 2016
  • The Baermann funnel method requires approximately four Kimwipe tissues for research a nematode count under a stereo microscope. To select more efficient and economical nematode extraction paper for nematode extraction, 15 different kinds of tissue papers were tested and compared with Kimwipe tissues. Nematode species used in the extraction efficiency tests include juvenile (J2) of Heterodera sp., J2 of Meloidogyne sp., Pratylenchus sp., Rhabditis sp., Acrobeloides sp., Panagrolaimus sp., Poikilolaimus sp. and Diplogasterida. The extraction efficiency varied between 42.0 to 88.8%. Considering costs, extraction efficacy, and clarity, the Pulling Kitchen Towel (Monalisa Co., Korea) is the best tissue, with clarity A, isolation efficiency of 69.4% (not significantly different compared to Kimwipe 1 ply 88.8%), and 1/2 costs per isolation of Kimwipe 2 ply.

Predatory Nematodes and Their Potential in Biological Control of Plant Parasitic Nematodes in Soil (포식선충의 토양중 식물기생선충의 생물학적 방제 이용 가능성)

  • Khan, Zakaullah;Kim, Young-Ho
    • The Korean Journal of Soil Zoology
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    • v.10 no.1_2
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    • pp.55-64
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    • 2005
  • Predatory nematodes are ubiquitous and feed on soil microorganisms including plant parasitic nematodes. They reduce populations of plant parasitic nematodes in virtually all soils because of their constant association with plant parasitic nematodes in the rhizosphere. Predatory potential of several species of predacious nematodes, belonging to the orders Mononchida, Diplogasterida, Dorylaimida and Aphelenchida, have been studied in detail on plant parasitic nematodes but most of the studies were based on in vitro experiments. A review of progress on the use of predatory nematodes as biological control agents of plant parasitic nematodes reveals that advocacy for predatory nematodes dates back to the early $20^{th}$ century; nevertheless, their potential has begun to be studied in recent years. Information on the efficacy of predatory nematodes under field conditions is lacking; however, some predatory nematodes have given very promising results against plant parasitic nematodes. This article summarizes research progress to date on predatory nematodes and discusses about their possible use in the management of plant parasitic nematodes.

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