Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of tetracycline-HCl root conditioning on gingival epithelial cell attachment to root surface

염산테트라싸이클린으로 처리한 치근면의 치은상피세포부착에 관한 연구

  • Hwang, Na-Young (Dept. of Periodontology College of Dentistry, Chosun University) ;
  • Park, Byung-Ki (Dept. of Periodontology College of Dentistry, Chosun University) ;
  • Kim, Sang-Mok (Dept. of Periodontology College of Dentistry, Chosun University) ;
  • Kuk, Jung-Ki (Dept. of Oral Biochemistry College of Dentistry, Chosun University) ;
  • Park, Ju-Chul (Dept. of Oral Histology College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Dept. of Periodontology College of Dentistry, Chosun University)
  • 황나영 (조선대학교 치과대학 치주과학교실) ;
  • 박병기 (조선대학교 치과대학 구강조직학교실, 구강생화학교실) ;
  • 김상목 (조선대학교 치과대학 구강조직학교실, 구강생화학교실) ;
  • 국중기 (조선대학교 치과대학 구강조직학교실, 구강생화학교실) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실, 구강생화학교실) ;
  • 김병옥 (조선대학교 치과대학 지주과학교실)
  • Published : 2002.03.30
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Abstract

The ultimate goal of periodontal therapy is directed to arresting the progression of the disease, and regenerating the fibrous attachment. In order to achieve such treatment aim, the plaque and calculus must be eliminated and the physiological conditions of the root surface must be changed to facilitate the attachment and migration of the new fibroblasts, The method of changing the proper root surface conditions to promote the healing of periodontal tissue involves mechanical procedures, such as scaling and root planing, and chemical procedures such as tetracycline-HCl. However, the formation of a long junctional epithelium was most frequently observed type of healing. Thus, the aim of this study was to examine in vitro the influence of surface conditioning of dentin by TC-HCl on human gingival epithelial cell attachment. Human gingival epithelial cells were obtained from healthy retromolar pad area(under the age 23 years). Seventy two teeth extracted from severe periodontitis were used as study material. To evaluate the epithelial cell attachment to dentin, the prepared specimen was divided to four groups. For the control group, only scaling and root planing were carried out, and for the test group, 1 to 3, the concentration of the TC-HCl was 50, 125 and 250mg/ml respectively. After cell cultivation time of 1-, 3-. 24 hour, for the indirect quantitative assessment of gingival epithelial cell attached to dentin sample, the absorbance of epithelial cell unattached to dentin was measured. The results were as follows; 1. There was no statistically significant difference between scaling and root planing group and TC-HCl 50mg/ml 125mg/ml and 250mg/ml group about absorbance of unattached epithelial cell to dentin sample(p>0.5). 2. As time passes, the absorbance of unattached gingival epithelial cell to dentin sample was decreased statistically significant(p<0.05). 3. There was no statistically significant difference among the TC-HCl group(p>0.05) We concluded that there was similar effect on gingival epithelial cell attachment between TC-HCl conditioning on root surface and only scaling and root planing treatment

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References

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