Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Lactococcus lactis Strain with ${\beta}$-Galactosidase Activity from Kimchi and Cloning of lacZ Gene from the Isolated Strain

  • Park, Rae-Jun (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Lee, Kwang-Hee (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim, Su-Jung (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Park, Jae-Yong (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Nam, Su-Jin (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Yun, Han-Dae (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, Seoul National University) ;
  • Chang, Hae-Choon (Department of Food and Nutrition, Chosun University) ;
  • Chung, Dae-Kyun (Institute and Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Jong-Hoon (Department of Foods and Biotechnology, Kyunggi University) ;
  • Park, Yun-Hee (Department of Molecular Science and Technology, Ajou University) ;
  • Kim, Jeong-Hwan (Division of Applied Life Science, Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University)
  • Published : 2002.02.01
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Abstract

A lactic acid bacteria with ${\beta}$-gal activity was isolated from Kimchi, a traditional fermented vegetable food in Korea. The isolate was identified as a Lactococcus lactis strain and named L. lactis A2. The gene encoding ${\beta}$-gal of L. lactis A2 was cloned as a 5.8 kb PstI fragment. DNA sequencing identified the complete lacA (galactoside acetyltransferase)-lacZ (${\beta}$-galactosidase) genes together with the 3' part of upstream galT (galactose-1-phosphate uridyltransferase), and the 5'region of downstream galE (UDP-galactose-4-epimerase) genes. L. lactis A2 had the same gal/lac operon structure as in L. lactis subsp. lactis 7962. Other genes of the Leloir pathway are most likely to be located in the 5'upstream of the 5.8 kb fragment on the A2 chromosome. Sequences downstream of galE were different from those of L. lactis subsp. lactis 7962.

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References

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