Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cellulase-Free Thermostable Alkaline Xylanase from Thermophilic and Alkalophilic Bacillus sp. JB-99

  • Naik, G.R. (Department of Biotechnology, Gulbarga University) ;
  • Johnvesly, B. (Department of Biotechnology, Gulbarga University) ;
  • Virupakshi, S. (Department of Biochemistry, Gulbarga University) ;
  • Patil, G.N. (Department of Biochemistry, Gulbarga University) ;
  • Ramalingam (Department of Biochemistry, Gulbarga University)
  • Published : 2002.02.01
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Abstract

The characterization of a partially purified, cellulase-free, thermostable alkaline xylanase from thermoalkalophilic Bacillus sp. JB-99 was investigated. The xylanase production was the highest when birchwood xylan was added to a medium containing finely powdered rice bran, showing 4,826 IU$ml^-1$ of activity for 15 h of incubation. The partially purified xylanase exhibited an optimum temperature and pH at $70^C{\circ}$ and 10, respectively. The enzyme was stable at pH 5-11 at $50^C{\circ}$. The xylanase activity was strongly inhibited by $Hg^2+$, while dithiothreitol, cysteine, and ${\beta}$-mercaptoethanol enhanced the activity.

Keywords

References

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