Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Expression of Periostin and S100A2 - S100A4 - Calcium Binding Proteins mRNA in Human Gingival Fibroblasts and Periodontal Ligament Fibroblasts

사람 치은섬유세포와 치주인대섬유모세포에서 Periostin과 S100A2-, S100A4-칼슘결합단백 mRNA의 발현

  • Kim, Byung-Ock (Dept. of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Han, Kyung-Yoon (Dept. of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Choi, Young-Sun (Dept. of Periodontology, College of Dentistry, Chosun University) ;
  • Kim, Se-Hoon (Dept. of Periodontology, College of Dentistry, Chosun University) ;
  • Park, Byung-Gi (Dept. of Periodontology, College of Dentistry, Chosun University) ;
  • Kim, Heung-Joong (Dept. of Oral Anatomy, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Park, Joo-Cheol (Dept. of Oral Histology, College of Dentistry, Chosun University, Oral Biology Research Institute)
  • 김병옥 (조선대학교 치과대학 치주과학교실, 구강생물학 연구소) ;
  • 한경윤 (조선대학교 치과대학 치주과학교실, 구강생물학 연구소) ;
  • 최용선 (조선대학교 치과대학 치주과학교실) ;
  • 김세훈 (조선대학교 치과대학 치주과학교실) ;
  • 박병기 (조선대학교 치과대학 치주과학교실) ;
  • 김흥중 (조선대학교 치과대학 구강해부학교실, 구강생물학 연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실, 구강생물학 연구소)
  • Published : 2001.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Gingival fibroblasts(GF) and periodontal ligament fibroblasts(PDLF) are the major cellular components of periodontal soft connective tissues, but the precise molecular biological differences between these cells are not yet known. In the present study, we investigated the expression of S100A4, S100A2 calcium-binding protein and osteoblast-specific factor 2(OSF-2, Periostin) mRNA in GF and PDLF in vitro through the process of reverse transcription-polymerase chain reaction(RT-PCR) and Northern blot analysis in each. Human GF and PDLF were isolated from the gingival connective tissue and the middle third of freshly extracted healthy third molars. They were cultured in Dulbecco's Modified Eagle Medium(DMEM) containing 10% fetal bovine serum and cells in the third passage were used in the experiments. After extracting total RNA from cultured cells, RT-PCR and Northern analysis were performed using S100A4-, S100A2- and Periostin-specific oligonucleotide primers and subcloned cDNA probes in each. In PT-PCR and Northern analysis, the expression of S100A4 and Periostin mRNA in GF was slightly detectable. Interestingly, the expression of S100A4 and periostin mRNA in PDLF was much higher than that in GF. On the other hand, S100A2 mPNA was highly expressed in both GF and PDLF. Since there was a marked difference of S100A4 and Periostin expression between GF and PDLF in vitro, these data suggest that S100A4 and periostin could be used as a useful marker for distinguishing cultured gingival fibroblasts and periodontal ligament cells.

Keywords