The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Central Localization of Neurons Projecting to the Uterus and Sanyinjiao(Sp6)

자궁과 삼음교에서 투사하는 충추신경계내 신경원의 표지영역에 관한 연구

  • 이창현 (우석대학교 한의과대학 해부학교실) ;
  • 강윤석 (우석대학교 한의과대학 한방병리학교실) ;
  • 이광규 (우석대학교 한의과대학 한방병리학교실) ;
  • 이상룡 (우석대학교 한의과대학 경혈학교실) ;
  • 육상원 (우석대학교 한의과대학 원전의사학교실)
  • Published : 2001.06.01
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Abstract

This experimental studies was to investigate location of labeled neurons in CNS following injection of pseudorabies virus(PRV), Bartha strain, into the uterus and Sanyinjiao(Sp6) of rats. After survival times of 4-5 days following the injection of PRV, the rats were perfused, and their brain and spinal cord were frozen sectioned($30\mu\textrm{m}$). These sections were stained by PRV immunohistochemical staining methods, and observed with light microscope. The results were as follows: 1. In the spinal cord, overlap areas of PRV labeled neurons projecting to uterus and Sp6 were observed in lamina VII, IX and X areas of cervical segments. In thoracic segments, overlap areas were observed in lamina IV, VII, X and intermediolateral n.. In lumbar segments, overlap area of PRV labeled neurons were observed in lamina I, V-VII, IX, X and intermediolateral n.. In sacral segments, overlap areas of PRY labeled neurons were observed in lamina N, V, VII, X and sacral parasympathetic n.. 2. In the brain, overlap areas of PR V labeled neurons projecting to the uterus and Sp6 were observed in lateral paragigantocellular n., rostroventrolateral reticular n., raphe obscurus n., raphe pallidus n., raphe magnus n., locus coeruleus n., Barrington's n., A5 cell group, central gray n., paraventricular hypothalamic n. and arcuate n. This results suggest that overlap areas of PRV labeled neurons of the spinal cord projecting to the uterus and Sp6 might be the first-order neurons related to the viscera-somatic sensory and sympathetic preganglionic neurons. PRV labeled neurons of the brain may be the second and third-order neurons response to the movement of smooth muscle of uterus. These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory and motor system monitoring the internal environment. These overlap areas of spinal cord and brainmay be related to autonomic centers related to regulation of uterus.

Keywords

References

  1. 경혈학(上) 전국한의과대학 침구경혈학교실(편저)
  2. Am. J. Chin. Med. v.4 Acupuncture loci. Gunn, G.C.
  3. Am. J. Chin. Med. v.8 Anatomical findings at acupuncture loci. Plummer, J.C.
  4. 경혈학 전국한의과대학 침구경혈학교실(편저)
  5. J. Comp. Neurol. v.140 Medullary cells of origin of vagal cardioinhibitory fibers in the pigeon. Cohen, D.H.;Schnell, A.M.;MacDonald, R.L.;Pitts, L.H.
  6. J. Comp. Neurol. v.208 A study of the extrinsic innervation of the guinea pig pylorus with the horseradish peroxidase tracing technique. Elfvin, L.G.;Lindh, B.
  7. Brain Res. v.491 A general pattern of CNS innervation of the sympathetic outflow demonstrated by transneuronal pseudorabies viral infections. Strack, A.M.;Sawyer, W.B.;Hughes, J.H.;Platt, K.B.;Loewy, A.D.
  8. Brain Res. v.616 Peripheral and central pathways regulating the kidney. a study using psudorabies virus. Schramm, L.P.;Strack, A.M.;Platt, K.B.;Loewy, A.D.
  9. 대한 침구학회지 v.17 대장과 관련된 경혈들의 신경 해부학적 연구 강창수;이상룡;이창현;남용재;이광규
  10. 석사학위논문 위와 족삼리(ST36)의 중추신경로 연결에 관한 연구 정한솔
  11. 中國鍼灸學 王德深
  12. 침구학 당옥수(外)
  13. 針灸學現代硏究與應用 郭長靑(外)
  14. J. Neurosci. v.226 Neurotropic properties od pseudorabies virus: uptake and transneuronal passage in the rat central nervous system. Card, J.P.;Rinaman, L.;Schwaber, J.S.;Miselis, R.R.;Whealy, M.E.;Robbins, A.K.;Enquist, L.W.
  15. 한방진단학 李鳳敎(外)
  16. 중의장상학 王琦(外)
  17. 삼음교 녹용약침이 백서의 난소적출로 변화된 女性 Hormone 및 골다공증에 미치는 영향 김선희
  18. Brain Res. Bull. v.25 Vagus nerveafferent and efferent innervation of the rat uterus : an electrophysiological and HRP study. Ortega-Villalobos, M.;Garcia-Bazan, M.;Solano-Flores, L.P.;Ninomiya-Alarcon, J.G.;Guevara-Guzman, R.;Wayner, M.J.
  19. J. Comp. Neurol. v.226 The localization and morphology of preganglionic neurons and the distribution of visceral afferents from therat pelvic nerve : horseradish peroxidase study. Nadelhaft, I.;Booth, A.M.
  20. 대한해부학회지 v.26 Psudorabies 바이러스를 이용한 흰쥐 子宮의 신경 지배에 관한 연구 이봉희;최완성;조경제;권해영;배지홍;송준경(외)
  21. J. Neurosci. v.13 Pseudorabies virus infection of the rat central nervous system : ultrastructural characterization of viral replication, transport and pathogenesis. Card, J.P.;Rinaman, L.;Lynn, R.B.;Lee, B.H.;Meade, R.P.;Miselis, R.R.;Enquist, L.W.
  22. Brain Res. v.506 Neurons in the sacral parasympathetic nucleus that project to the hypothalamus do not also project through the pelvic nerve : a double labeling study combining Fluoro-Gold and cholera toxin B in the rat. Burstein, R.;Wang, J.;Elde, R.P.;Giesler, G. Jr.
  23. Anat. Rec. v.241 Parasympathetic preganglionic innervation are cholinergic and nitric oxide-containing. Papka, R.E.;McCurdy, J.R.;Williams, S.J.;Mayer, B.;Marson, L.;Platt, K.B.
  24. Neurosci. Lett. v.143 Central nervous systemneurons labeled following the injection of pseudorabies virus into the rat urinary bladder. Nadelhaft, I.;Vera, P.L.;Card, J.P.;Miselis, R.R.
  25. J. Comp. Neurol. v.389 Identification of central nervous system neurons thatinnervate the bladder body, bladder base, or external urethral sphincter of female rats : a transneuronal tracing study using pseudorabies virus. Marson, L.
  26. J. Comp. Neurol. v.183 A preganglionic autonomic nucleus in the dorsal gray commissure of the lumbar spinal cord of the rat. Hancock, M.B.;Peveto, C.A.
  27. Exp. Brain Res. v.98 Thoracolumbar ympathetic preganglionic neurones in the dorsal commissural nucleus of the malerat : an immunohistochelical stusy using retrograde labeling of choleratoxin subunit B. Hosoya, Y.;Nadelhaft, I.;Wang, D.;Kohno, K.
  28. J. Comp. Neurol. v.256 Sexual dimorphism in sympathetic preganglionic neurons of the rat hypogastric nerve. Nadelhaft, I.;McKenna, K.E.
  29. Brain Res. v.172 Descending projections from the pontine micturition center. Loewy, A.D.;Saper, C.B.;Baker, R.P.
  30. Brain Res. v.592 Lesions of the nucleus paragigantocellularis alter ex-copula penile reflexes. Marson, L.;List, M.S.;McKenna, K.E.
  31. Neuroscience. v.74 Endogenous opiods acting at a medullary-opiod receptor contribute to the behavioral antinociception produced by GABA antagonism in the midbrain periaqueductal gray. Roychowdhury, S.M.;Fields, H.L.
  32. Brain Res Brain Res. Rev. v.3 Efferent connections of the ventral medulla oblongata in the rat. Loewy, A.D.;Wallach, J.H.;McKellar, S.
  33. J. Comp. Neurol. v.226 Localization of forebrain neurones which project directly to the medulla and spinal cord of the rat by retrograde tracing with wheat germ agglutinin. Schwanzel-Fukuda, M.;Morel, J.I.;Pfaff, D.W.
  34. Brain Res. v.539 Projections of the paraventricular nucleus of the hypothalamus to the sexually dimorphic lumbosacral region of the spinal cord. Wagner, C.K.;Clemens, L.G.
  35. Exp. Brain Res. v.85 Descending input from the hypothalamic paraventricular nucleus to sympathetic preganglionic neurons in the rat. Hosoya, Y.;Sugiura, Y.;Okado, N.;Loewy, A.D.;Kohno, K.
  36. Brain Res. v.343 A method for anterograde axonal tracingof chemically specified circuits in the central nervous system : combined phaseolus vulgaris-leucoagglutinin(PHA-L) tract tracing and immunohis tochemistry. Gerfen, C.R.;Sawchenko, P.E.