Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Testing of Cancer Chemopreventive Potential of Prunella vulgaris L. Aqua-acupuncture Solution Using Biochemical Markers of Carcinogenesis

발암과정 생화학적 표식자를 이용한 하고초 약침액의 암예방 활성 측정

  • Park, Sin-Hwa (Department of AM-Pointology, College of Oriental Medicine) ;
  • Cho, Kyoung-Hee (Department of AM-Pointology, College of Oriental Medicine) ;
  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Diseases Research Center, Dongguk University) ;
  • Lim, Jong-Kook (Department of AM-Pointology, College of Oriental Medicine) ;
  • Nam, Kyung-Soo (Department of AM-Pointology, College of Oriental Medicine)
  • 박신화 (동국대학교 한의과대학 경혈학교실) ;
  • 조경희 (동국대학교 한의과대학 경혈학교실) ;
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실) ;
  • 남경수 (동국대학교 한의과대학 경혈학교실)
  • Published : 2001.06.30
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Abstract

Prunella vulgaris L. aqua-acupuncture solution (PVAS) was tested for cancer chemopreventive activity using chemoprevention-associated biochemical end points. The following effects were measured. : (a) inhibition of 7,12-dimethylbenz[a]anthracene (DMBA)-induced cytochrome P4501A1 activity (b) inhibition of $[^3H]B[a]P-DNA$ binding (c) inhibition of phorbol 12-myristate 13-acetate (TPA)-induced free radical formation in HL-60 cells (d) inhibition of polyamine metabolism. PVAS inhibited cytochrome P4501A1-mediated ethoxyresorufin-O-deethylase activity. The binding of $[^3H]B[a]P$ metabolites to DNA of NCTC-clone 1469 cells was inhibited significantly by PVAS. There is 22% inhibition of TPA-induced free radical formation in human leukemic cells with 5 mg/ml PVAS. Proliferation of Acanthamoeba castellanii was inhibited by PAVS at concentration of 30 mg/ml. PAVS positive in these assays may inhibit the carcinogenesis process and is considered very promising cancer-preventing agent because of its multiple activities.

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