The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Effects of Sebsaeng-eum(Shesengyin) on the NO Production of $C_6$ Glial Cell

섭생음이$C_6$ glial 세포의 NO 생성에 미치는 영향

  • 임창용 (원광대학교 한의과대학 심계내과학교실, 원광대학교 부속광주한방병원 심계내과) ;
  • 김요한 (원광대학교 한의과대학 심계내과학교실, 원광대학교 부속광주한방병원 심계내과) ;
  • 박세홍 (원광대학교 한의과대학 심계내과학교실, 원광대학교 부속광주한방병원 심계내과) ;
  • 이소영 (원광대학교 한의과대학 심계내과학교실, 원광대학교 부속광주한방병원 심계내과) ;
  • 이상관 (원광대학교 한의과대학 심계내과학교실, 원광대학교 부속광주한방병원 심계내과) ;
  • 성강경 (원광대학교 한의과대학 심계내과학교실, 원광대학교 부속광주한방병원 심계내과)
  • Published : 2000.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The water extract of Sebsaeng-eum(SheShengYin) has been used for treatment of ischemic brain damage in oriental medicine, However, little is known about the mechanism by which the water extract of Sebsaeng-eum(SheShengYin) rescues brain cells from ischemic damages. Methods : To elucidate the protective mechanism on ischemic induced cytotoxicity, We investigated the regulation of LPS and PMA induced iNOS expression in $C_{6}$ glial cells. Results : LPS and PMA treatment for 48 h in $C_{6}$ glial cells markedly induced NO, but treatment of the cells with the water extract of Sebsaeng-eum(SheShengYin) decreased nitrite formation. In addition, LPS and PMA treatment for 48 h induced severe cell death in $C_{6}$ glial cells. However treatment of the cells with the water extract of Sebsaeng-eum(SheSheng Yin) did not induce significant changes compared to the control. LPS and PMA induced iNOS activation in $C_{6}$ glial cells caused chromosomal condensation and fragmentation of nuclei. Conclusions : Taken together, We suggest that the protective effects of the water extract of Sebsaeng-eum(SheShengYin) against ischemic brain damages may be mediated by regulation of iNOS during ischemic condition.

Keywords

References

  1. 萬病回春 공정현
  2. 校訂方劑大辭典 江克明;包明惠
  3. 東醫寶鑑 許浚
  4. 醫部全錄 陳夢雷
  5. 四庫全書 第七六六冊 印景;文淵閣
  6. 診療要鑑 金定濟
  7. 東醫臨床處方集 東醫臨床處方集編纂學生委員會
  8. 古代驗方大全 傳景華
  9. 東醫處方大全 東醫科學院
  10. 東醫學辭典 과학백과사전종합출판사
  11. 幼幼集成 陳復正
  12. 心系內科學 全國韓醫科大學 心系內科學敎室
  13. 東醫內科學 具本泓
  14. 신경외과학 대한신경외과학회
  15. 백서 대뇌 피질,해마와 선조체 절편에서 허혈의 神經 전도 물질 유리효과 金東燦
  16. 신경학원론 서울대학교의과대학
  17. 實用中醫內科學 方藥中(외)
  18. 中醫腦病學 許沛虎
  19. 定志丸이 腦組織의 生化學的 變化와 神經細胞의 損傷에 미치는 실험적 硏究 최용준
  20. 太陰人 淸心蓮子湯이 Hydrogen Peroxide에 損傷된 白鼠의 大腦神經細胞에 미치는 影響 玉潤榮
  21. 原色臨床本草學 辛民敎
  22. Proc. Natl. Acad. Sci. USA. v.88 Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures Dawson, V.L.;Dawson, T.M.;London, E.D.;Bredt, D.S.;Snyder, S.H.
  23. J. Neurosci. v.13 Mechanism of nitric oxide-mediated neurotoxicity in primary brain cultures Dawson, V.L.;Dawson,T.M.;Bartley,D.A.;Uhl, G.R.;Snyder, S.H.
  24. J.Chem.Neuroanat. v.10 Nirtic oxide neurotoxicity Dawson, V.L.;Dawson, T.M.
  25. Experientia v.49 Towards an understanding of the role of glutamate in neurodegenerative disorders.energy metabolism and neuropathology Turski,L.;Turski,WA.
  26. Curr.Opin.Neurol. v.8 Excitotoxicity and neurodeginerattive diseases Ikonomidou,C.;Turski,L.
  27. Curr. Opin. Neurol. v.8 Role of nitric oxide in neurodegenerative diseases Schulz,J.B.;Matthews,R.T.;Beal,M.F.
  28. Nature Endothelium- derived relaxing factor release on activation of NMDA receptors suggests a role as intercellular messanger in the brain Garthwaite, J.;Chales, S.L.;Chess-Williams, R.
  29. Progr. Neurobiol. v.42 Nitric oxide : a radical neurotransmitter in the central mervous system Vincent, S.R.
  30. Proc.Natl.Acad.Sci.USA. v.87 Isolaion of niric oxide synhease, a calmodulin- requiring enzyme Bredt, D.S.;Snyder, S.H.
  31. Biochem. J. v.298 Nitric oxide synhases in mammals Knowles, R.G.;Moncada, S.
  32. Am. J. Physiol. v.42 NO contributes to neurohypophysial but not other regional cerebral fluorocarbon-induced hyperemia in cats Wagner, B.P.;Stingele, R.;Williams, M.A.;Wilson, D.A.;Traystman, R.J.;Hanley,D.F.
  33. Biochem. Biophys. Acta. v.1181 EPR spintrapping study of intric-oxide formation during bilateral carotid occlusion in the rat Sato, S.;Tominaga, T.;Ohnishi, S.T.
  34. Brain Res. v.614 Potentiation of nitric oxide formation following bilateral carotid occlusion and focal cerebral-ischemia in the rat. In vivo detection of nitric oxide radial by electron-paramagnetic-resorance spin trapping Tominaga, T.;Sato, S.;Ohnishi, S.T.
  35. Biochimica. Biopysica. Acta. v.1141 Roles of nitric oxide in brain hypoxia-ischemia paper. Juan P.B.;Angeles A.
  36. Stroke v.27 Inducible nitric oxide synthase gene-expression in vascular cells after transient focal cerebral-ischemia Iadecola, C.;Zhang, R.Y.;Casey, R.;Clark, H.B.;Ross, M.E.
  37. J Neurochem. v.71 no.5 Nonsteroid -al anti-inflammatory drugs increase tumor necrosis factor production in the periphery but not in the central nervous system in mice and rats Sacco S.;Agnello D.;Sottocorno M.;Lozza G.;Monopoli A.;Villa P.;Ghezzi P.
  38. J.Cereb. BloodFolw Metab. v.15 Marked induction of Calcium-independent nitric-oxide synthase activity after focal cerebral-ischemia Iadecola, C.;Xu, X.H.;Ahang, F.Y.;Elfakahany, E.E.;Rose, M.E.
  39. Am. J. Physiol. v.37 Inhibition of inducible nitric-oxide synthase ameliorates cerebral ischemic damage Iadecola, C.;Zhang, F.Y.;Xu, X.H.
  40. J Mol Cell Cardiol. v.29 no.9 Protection by inhibition of poly(ADP-ribose) synthetase against oxidant injury in cardiac myoblast In vitro Gilad, E.;Zingarelli B.;Salzman AL.;Szabo C.