Journal of fish pathology (한국어병학회지)
- Volume 13 Issue 1
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- Pages.61-66
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- 2000
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- 1226-0819(pISSN)
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- 2233-5412(eISSN)
A Simple Method for the Concentration of Fish Pathogenic Virus in Sea Water
한외여과막을 이용한 해수내 어류 병원바이러스 농축법
- Oh, Myung-Joo (Department of Fish Pathology, Yosu National University) ;
- Kim, Suk-Ryul (Department of Fish Pathology, Yosu National University) ;
- Jung, Sung-Ju (Department of Fish Pathology, Yosu National University) ;
- Kim, Hyeung-Rak (Department of Food Science & Biotechnology, Pukyong National University) ;
- Kim, Heung-Yun (Department of Fish Pathology, Yosu National University) ;
- Yeo, In-Kyu (Department of Marine Biotechnology, Cheju National University)
- 오명주 (여수대학교 어병학과) ;
- 김석렬 (여수대학교 어병학과) ;
- 정성주 (여수대학교 어병학과) ;
- 김형락 (부경대학교 식품생명과학) ;
- 김흥윤 (여수대학교 어병학과) ;
- 여인규 (제주대학교 해양생물공학과)
- Published : 2000.05.30
Abstract
A method was developed for concentrating fish pathogenic virus from sea water using membrane ultrafiltration system and centricon. The method consists of passing large volumes (Ca. 20 liter) of sea water through ultrafiltration (PAN) filter followed by cross-flow filtration method and centrifugation use the centricon (Plus-20). This procedure permitted the processing of 20 liter of sea water which resulted in a 20,000-fold reduction in the volume of water and greater than 90% recovery of the seeded MABV.
해수중에 소수로 분포하는 어류 병원 바이러스의 검출을 목적으로 해산어류 병원바이러스의 일종인 marine birnavirus (MABV)를 대상으로하여 한외 여과막과 centricon을 이용한 농축효과를 바이러스의 주화세포내 감염에 의해 형성되어지는 CPE (cytopathic effect) 출현에 근거한 바이러스 감염가 측정법인