Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 14 Issue 1
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- Pages.59-67
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- 1999
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Quantitative Selection of Capacitated-sperm with BSA, Caffeine, Heparin and Progesterone
BSA, Caffeine, Heparin 및 Progesteron을 이용한 수정능획득정자의 양적 선별
Abstract
To make up the medium for quantitative selection of capacitated-sperm through sucrose layer, the effects of BSA, caffeine, heparin and progesterone on sperm swim-up migration and movement were examined. And the results obtained were as follows; 1. BSA of 4mg/ml in bMSS stimulated sperm migration and movement, and attracted capacitated-sperm. 2. Caffeine of 5mM in bMSS containing 4mg/ml BSA stimulated sperm movement and attracted capacitated-sperm. 3. Heparin of 20
Keywords
- BSA;
- caffeine;
- heparin;
- progesterone;
- sperm selection;
- swim-up migration;
- movement;
- capacitation;
- sucrose layer