Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 14 Issue 1
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- Pages.39-46
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- 1999
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Embryo Transfer and Establishment of the Optimal Culture Systems for Production of Good Quality Blastocyst Derived In Vitro in Korean Cattle
체외에서 양질의 한우 수정란 생산을 위한 배양조건의 설정 및 이식
Abstract
To establish the optimal culture systems for production of transferable embryos in Korean Cattle, pregnancy rates of IVF-derived blastocysts according to different culture media, culture method and culture duration were compared. Development of IVF-derived embryos to blastocysts was most effective in YS medium group co-cultre with cumulus cells. Blastocysts cultured for 6 to 8 d in vitro showed higher hatching rate and good quality. Pregnancy rates after transfer of IVF-derived blastocysts cultured for 7 or 8 d were high. Through our experiments, it is considered that improvement of culture media and culture method is necessary for mass production of blastocysts with excellent of good quality in Korean Cattle.