Journal of Microbiology

  • 제37권1호
  • /
  • Pages.35-40
  • /
  • 1999
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Molecular Cloning and Analysis of Sporulation-Specific Glucoamylase (SGA) Gene of Saccharomyces diastaticus

  • Kang, Dae-Ook (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon) ;
  • Hwang, In-Kyu (The Scripps Institute, La Jolla CA, USA) ;
  • Oh, Won-Keun (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon) ;
  • Lee, Hyun-Sun (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon) ;
  • Ahn, Soon-Cheol (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon) ;
  • Kim, Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon) ;
  • Mheen, Tae-Ick (The Scripps Institute, La Jolla CA, USA) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon)
  • 발행 : 1999.03.01
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초록

Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.

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