Journal of Microbiology

  • 제37권1호
  • /
  • Pages.27-34
  • /
  • 1999
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Microscopic Examination of the Suppressive Action of Antifungal Substances from Pseudomonas aeruginosa on Asexual Sporulation of Fungi

  • Kwon S. Yoon (Department of Microbiology, Kangwon National University, Chunchon) ;
  • Bu Y. Min (Department of Microbiology, Kangwon National University, Chunchon) ;
  • Park, Hyoung T. (Department of Microbiology, Kangwon National University, Chunchon) ;
  • Lee, Jong K. (Department of Forest Resources Protection, Kangwon National University, Chunchon, Kore) ;
  • Kim, Kun W. (School of Bioresources, Andong National University, Andong)
  • 발행 : 1999.03.01
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초록

Two fractions with unusual antifungal activity that suppress asexual sporulation of several fungi were obtained from culture filtrate of Pseudomonas aeruginosa and were partially purified through the repeated silicagel flash column chromatographies. The sporulation-suppressive actions of these fractions in Aspergillus nidulans, Rhizopus stolonifer, and Coprinus cinereus, were analyzed by light and electron microscopes. The germination ability of the spores produced in the presence of these fractions were also checked to determine the persistent effects of these antifungal substances on the next generation. Light microscopic observation of developing sporangia of R. stolonifer grown in the presence of both fractions revealed that the significant number of sporangia failed to reach maturity, and frequently, uncontrolled growths of hyphae and rhizoids from the sporangiophores were found. In A. nidulans addition of these fractions appeared to cause different classes of morphological abnormality in conidia development, which included aborted formation of conidiogenous cells from the apex of conidiophores and enhanced hyphal growths either at the tip or middle of the conidiophores. Germination abilities of spores obtained from the cultures grown in the presence of antifungal fractions were 40∼60% in Aspergillus, 50∼80% in Coprinus (thallic spores), and 30∼40% in Rhizopus compared to those of normal spores.

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참고문헌

  1. Microbiol. Immunol. v.38 Characterization of hemolytic and antifungal substance, cepalycin, from Pseudomonas cepacia Abe, M.;T. Nakazawa
  2. Plant Pathol. v.37 Mechanism of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads Brisbane, P.G.;A.D. Rovira
  3. Soil Biol. Biochem. v.8 The role of bacteria in the biological control of Gaeumannomyces graminis by suppressive soils Cook, R.J.;A.D. Rovira
  4. Trends in Biotechnol. v.12 Metabolites of Pseudomonas involved in the biocontrol of plant disease Dowling, D.N.;F. O'Gara
  5. Appl. Environ. Microbiol. v.58 Exploitation of gene(s) involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain Fenton, A.M.;P.M. Stephens;J. Crowley;M. O'Callahaghan;F. O'Gara
  6. J. Bacteriol. v.170 An iron-antagonized fungistatic agent that is not required for iron assimilation from a fluorescent rhizosphere pseudomonad Gill, P.R.;G.H. Warren
  7. Critical Rev. Biotechnol. v.10 Microbial fungicides: Recent approaches to elucidating mechanisms Gutterson, N.
  8. Appl. Environ. Microbiol. v.57 Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R Hamdan, H.;D.M. Weller;L.S. Thomashow
  9. Kor. J. Pl. Pathol. v.4 Studies on the biological control of Phytophthora blight of red-pepper. Ⅰ. Isolation of antagonists and evaluation of antagonistic activity in vitro and green house Jee, H.H.;C.K. Nam;C.H. Kim
  10. Mol. Pl-Microbe Interact. v.5 Suppression of root diseases by Pseudomonas fluorescens CHA0: Importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol Keel, C.;U. Schnider;M.Maurhofer;C. Voisard;J. Laville;U. Burger;P. Wirthner;D. Haas;G. Defago
  11. Kor. J. Pl. Pathol. v.7 Isolation and identification of antibiotic substances produced by Pseudomonas fluorescens Kim, J.S.;Y.W. Lee;S.W. Oh;Y.K. Yi;Y.H. Yu;Y.H. Kim;K.J. Park
  12. Kor. J. Pl. Pathol. v.6 Studies on biological control of Phytophthora blight of red pepper. Ⅳ. Performance of antagonistic agents in field under polyethylene film house Lee, E.J.;H.J. Jee;K.S. Park;C.H. Kim
  13. Kor. J. Pl. Pathol. v.6 Evaluation of biocontrol activity of fluorescent pseudomonads against some rice fungal diseases in vitro and green house Lee, Y.H.;G.Y. Shim;E.J. Lee;T.W. Mew
  14. Appl. Environ. Microbiol. v.58 Pseudomonas cepacia suppression of sunflower wilt fungus and role of antifungal compounds in controlling the disease McLoughlin, T.J.;J.P. Quinn;A. Betterman;R. Bookland
  15. J. Microbiol. v.34 Fungal-sporulation suppressing substances produced by Pseudomonas aeruginosa Min, B.Y.;J.Y. Shim;K.W. Kim;J.K. Lee;H.T. Choi;K.S. Yoon
  16. Kor. J. Pl. Pathol. v.6 Biological control of Fusarium wilt of strawberry by antagonistic bacterium, Pseudomonas gladioli, in greenhouse Moon, B.J.;S.H. Roh;C.T. Cho
  17. Microbiol. Rev. v.56 Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens O'Sullivan, D.J.;F. O'Gara
  18. Appl. Microbiol. Biotechnol. v.44 Improved delivery of biocontrol Pseudomonas and their antifungal metabolites using alginate polymers Russo, A.;Y. Moenne-Loccoz;S. Fedi;P. Higgins;A. Fenton;D.N. Dowling;M. O'Regan;F. O'Gara
  19. J. Bacteriol. v.177 Amplification of the house-keeping sigma factor in Pseudomonas fluorescence CHA0 enhances antibiotic production and improves biocontrol abilities Schnider, U.;C. Keel;C. Blumer;J. Troxler;G. Defago;D. Haas
  20. Appl. Environ. Microbiol. v.58 Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonads and investigation of physiological parameters influencing its production Shanahan, P.;D.J. O'Sullivan;P. Simpson;J.D. Glennon;F. O'Gara
  21. J. Antibiotics v.41 Isolation of a new phenazine antibiotic, DOB-41, from Pseudomonas species Shoji, J.;R. Sakazaki;H. Nakai;Y. Terui;T. Hattori;O. Shiratori;E. Kondo;T. Konishi
  22. J. Antibiotics v.43 Isolation of cepafungins Ⅰ,Ⅱ, and Ⅲ from Pseudomonas species Shoji, J.;H. Hino;T. Kato;T. Hattori;K. Harooka;K. Tawara;O. Shiratori;Y. Terui
  23. J. Bacteriol. v.170 Role of phenazine antibiotics from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var Thomashow, L.;D.M. Weller
  24. J. Antibiotics v.44 Isolation and characterization of phosmidosine a new antifungal nucleotide antibiotic Uramoto, M.;C.J. Kim;K. Shin-Ya;H. Kusakabe;K. Isono
  25. Appl. Environ. Microbiol. v.57 Genetic analysis of the antifungal activity of soil-borne Pseudomonas aureofaciens strain Vincent, M.N.;L.A. Harrison;J.M. Brackin;P.A. Kovacevich;P. Mukerji;D.M. Weller;E.A. Pierson
  26. Gene v.168 The phzI gene of Pseudomonas aureofaciens 30-84 is responsible for the production of diffusible signal required for phenazine antibiotic production Wood, D.W.;L.S. Pierson Ⅲ