Journal of Microbiology

  • 제37권1호
  • /
  • Pages.41-44
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  • 1999
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Expression of Human Mitochondiral Aldehyde Dehydrogenase 2 in Mammalian Cells using Vaccinia Virus-T7 RNA Polymerase

  • 발행 : 1999.03.01
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초록

Human mitochondrial aldehyde dehydrogenase 2 (ALDH2) is mainly responsible for oxidation of acetaldehyde generated during alcohol oxidation in vivo. A full-length cDNA of human liver ALDH2 was successfully expressed using a vaccinia virus-T7 RNA polymerase system. The expressed ALDH2 had an enzymatic activity as high as the native human liver ALDH2 enzyme.

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참고문헌

  1. Molecular cell biology(3rd ed.) Darnell, J.;H. Lodish;D. Baltimore;Berk, A.
  2. Adv. Exp. Med. Biol. v.372 Tissue-specific expression and preliminary functional analysis of the 5' flanking region of the human mitochondrial aldehyde dehydrogenase (ALDH2) gene Dipple, K.M.;M.J. Stewart;D.W. Crabb
  3. Proc. Natl. Acad. Sci. USA v.83 Eukarotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase Fuerst, T.R.;G. Edward;F. Niles
  4. Proc. Natl. Acad. USA v.83 Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase Fuerst, T.R.;E.G. Niles;F.W. Studier;B. Moss
  5. Clin. Genet. v.16 Alcohol metabolizing enzymes: Studies of isoenzymes in human biopsies and cultured fibroblast Goedde, H.W.;D.P. Agarwal;S. Harada
  6. J. Biol. Chem. v.267 Human stomach aldehyde dehydrogenase cDNA and genomic cloning, primary structure, and expression in Escherchia coli Hus, L.C.;W.C. Chang;A. Shibuya;A. Yoshida
  7. J. Biol. Chem. v.267 Specific binding sites for the activator protein, ALCR in the alca promoter of the ethanol regulation of Aspergullus Nidulans Kulumburg, P.;N. Judewicz
  8. Korean J. Food & Nutr. v.10 Expression of human ALDH2 in Escherichia coli Kwak, B.Y.;K.H. Lee;H.S. Jeong
  9. Alcohol. Clin. Exp. Res. v.21 Genetic polymorphism of cytochrome P450 2E1 and mitochondrial aldehyde dehydrogenase in a korean population Lee, K.H.;B.Y. Kwak;J.H. Kim;S.K. Yoo;S.K. Yum;H.S. Jeong
  10. J. Biol. Chem. v.259 Rat liver aldehyde dehyddrogenase isolation and characterization of four high Km normal liver isozymes Lindahl, R.;S. Eveces
  11. Alcohol Alcohol v.29 Involvement of alcohol metabolism Mizoi, Y.;K. Yamamoto;Y. Ueno;T. Fukunaga;S. Harada
  12. Biochem. Pharmacol. v.45 Nitroxyl analogs as inhibitors of aldehyde dehydrogenase C-nitroso compounds Nagasawa, H.T.;Y. Yost;J.A. Elberling;F.N. Shirota;E.G. Demaster
  13. Hum. Genet. v.82 Genotype of alcohol dehydrogenase and aldehyde dehydrogenase loci in Japanese alcohol flushers and non-flushers Shibuya, A.;M. Yasunami;A. Yoshida
  14. Biochem. J. v.135 The subcellular distribution and properties of aldehyde dehydrogenase in rat liver Tottmar, S.O.;H. Pettersson;K.H. Kiessling
  15. J. Clin. Invest. v.96 The Aldehyde dehydrogenase ALDH2*2 allele exhibits dominance over ALDH2*1 in transduced HeLa cells Xiao, Q.;H. Weiner;T. Johnston;D.W. Crabb
  16. Prog. Nucleic Acid Res. Mol. Biol. v.40 Genetics of human alcohol metabolizing enzymes Yoshida, A.;L.C. Hsu;M. Yasunami
  17. Alcohol. Clin. Exp. Res. v.17 Cloning and expression of the full-length cDNAs encoding human liver class1 and class2 aldehyde dehydrogenase Zheng, C.F.;T.Y. Thomas;H. Weiner
  18. Eur. J. Biochem. v.245 Binding of thyroxine analogs to human liver aldehyde dehydrogenases Zhou, J.;H. Weiner