The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Characterization of a Wound or UV Induced cDNA Fragment from Pleurotus sajor-caju

상처 및 자외선 자극에 반응하는 여름느타리 cDNA 단편의 분리 및 그 발현 특성

  • Park, Soo-Chul (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Jung, Uk-Jin (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Jeong, Mi-Jeong (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Bum-Gi (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Yoo, Young-Bok (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Ryu, Jin-Chang (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA)
  • 박수철 (농업과학기술원 분자유전과) ;
  • 정욱진 (농업과학기술원 분자유전과) ;
  • 정미정 (농업과학기술원 분자유전과) ;
  • 김범기 (농업과학기술원 분자유전과) ;
  • 유영복 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과)
  • Published : 1998.09.30
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Abstract

A 0.4 kb cDNA fragment was isolated from mRNA of UV or mechanical wound damaged Pleurotus sajor-caju by the differential display method. Expression of the gene corresponding to this cDNA fragment was highly induced by mechanical wound damage or UV treatment. This gene showed only basal level expression in mycelia, stipe, and cap under normal growth conditions. Sequencing analysis showed that this cDNA fragment contains partial open reading frame. Homology search using genbank database revealed that although this gene do not have homology with already reported wound induced genes, it has a significant sequence homology in defined region with the cdc2-related protein kinase gene which is known to be involved in negative regulation of meiotic maturation in Xenopus oocytes.

mRNA의 differential display방법에 의해 여름느타리 자실체의 상처 또는 자외선 처리시 발현되는 약 0.4kb의 cDNA 단편을 분리하였다. 이 cDNA 단편의 염기서열 분석결과 세포분열 촉진에 관여하는 cdc2-related protein kinase gene과 상당부분 유사성을 보였으며 RT-PCR 방법을 이용한 분리 유전자의 발현 실험을 통해 이 유전자가 정상 생장 환경에서는 갓, 대, 균사 등 모든 부위에서 기본적인 발현상태를 유지하고 있으며 기계적 상처 또는 자외선 처리에 의해 그 발현이 증폭됨을 확인하였다. 이러한 결과를 통해 향후 분리된 유전자의 연구를 통한 버섯 병 방어 관련 신호 전달 체계 분석에 대한 가능성을 검토해 보았다.

Keywords

References

  1. Annu. Rev. Genetics v.29 The plant response in pathogenesis, symbiosis, and wounding: Variations on a common theme Baron, C.;Zambryski, P.C.
  2. Growth media. more gene manipulations in fungi Bennett, J.W.;Lasure, L.L.
  3. Mol. Cell. Biol. v.14 Characterization of cis-acting elements in light regulation of the nuclear gene encoding the A subunit of chloroplast isozymes of Glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana Conley, T.R.;Park, S.C.;Kwon, H.B.;Peng, H.P.;Shih, M.C.
  4. Proc. Natl. Acad. Sci. USA v.92 Systemin activates synthesis of woundinducible tomato leaf polyphenol oxidase via the octadecanoid defense signalling pathway Constabel, C.P.;Bergey, D.R.;Ryan, C.A.
  5. Biol. Rev. v.61 The phytoalexin response: Elicitin, signalling and control of host gene expression Dixon, R.A.
  6. Annu. Rev. Plant Physiol. Plant Mol. Biol. v.41 Molecular communication in interactions between plants and microbial pathogens Dixon, R.A.;Lamb, C.J.
  7. Annu. Rev. Phytopathol. v.32 Early events in the activation of plant defense responses Dixon, R.A.;Harrison, M.J.;Lamb, C.J.
  8. Plant J. v.7 Systemin induces rapid ion fluxes and ethylene biosynthesis in Lycopersicon peruvianum cells Felix, G.;Boller, T.
  9. Biosynthesis and biodegradation of wood component Higuchi, T.
  10. Plant Physiol. v.86 Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding, infection Hedrick, S.A.;Bell, J.;Boller, N.T.;Lamb, C.J.
  11. Plant Physiol. v.115 Evidence for chewing insect-specific molecular events distinct from a general wound response in leaves. Korth, K.L.;Dixon, R.A.
  12. Mol. Cell. Biol. v.7 Transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection Lawton, M.A.;Lamb, C.J.
  13. Science v.257 Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction Liang, P.;Pardee, A.B.
  14. Proc. Natl. Acad. Sci. USA v.94 Jasmonate is essential for insect defense in Arabidopsis McConn, M.;Creelman, R.A.;Bell, E.;Mullet, J.E.;Browse, J.
  15. Science v.255 Structure, expression and antisense inhibition of the systemin precursor gene MeGurl, B.;Pearce, G.;Orozco-Cardenas, M.L.;Ryan, C.A.
  16. EMBO J. v.6 Chalcone isomerase cDNA cloning and mRNA induction by fungal elicitor, wounding, and infection Mehdy, M.C.;Lamb, C.J.
  17. Proc. Natl. Acad. Sci. USA v.90 Expression of an antisense prosystemin gene in tomato reduces resistance toward Manduca sexta larvae Orozco-Cardenas, M.L.;McGurl, B.;Ryan, C.A.
  18. Proc. Natl. had. Sci. USA v.92 Signals involved in wound-induced proteinase inhibitor II gene expression in tomato and potato plants Pena-Cortes, H.;Fisahn, J.;Willmitzer, L.
  19. Plant Cell v.9 Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to severe bacterial pathogens and alterations in PR-1 gene expression Rogers, E.E.;Ausubel, F.M.
  20. Annu. Rev. Phytopathol. v.28 Protease inhibitors in plants: Genes for improving defenses against insects and pathogens Ryan, C.A.
  21. Molecular cloning: a laboratory manual Sambrook, J.;Fritsch, E.F.;Maniatis, T.
  22. EMBO J. v.9 no.10 p40M015, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes Shuttleworth, J.;Godfrey, R.;Colman, A.
  23. The Plant Journal v.2 Rapid analysis of plant gene expression by a novel reverse transcriptase PCR method Simpson, C.G.;Sinibaldi, R.;Brown, J.W.S.
  24. Plant Cell v.3 Coordinate gene activity in response to agents that induce systemic acquired resistance Ward, E.R.;Uknes, S.J.;Williams, S.C.;Dincher, S.S.;Wiederhold, D.L.;Alexander, D.C.;Ahi-Goy, P.;Metraux, J.P.;Ryals, J.A.
  25. Nature v.360 Electrical signalling and systemic proteinase inhibitor induction in the wounded plant Wildon, D.C.;Thain, J.F.;Minchin, P.E.H.;Gubb, I.R.;Reilly, A.J.;Skipper, Y.D.;Doherty, H.M.;O'Donnell, P.J.;Bowles, D.J.